Immunosensor for diagnosis of Alzheimer disease using amyloid-beta 1-40 peptide and silk fibroin thin films

dc.creatorGoncalves, J. M.
dc.creatorLima, L. R.
dc.creatorMoraes, Marli Leite de [UNIFESP]
dc.creatorRibeiro, S. J. L.
dc.date.accessioned2020-07-31T12:47:31Z
dc.date.accessioned2022-10-07T21:19:39Z
dc.date.available2020-07-31T12:47:31Z
dc.date.available2022-10-07T21:19:39Z
dc.date.created2020-07-31T12:47:31Z
dc.date.issued2016
dc.identifierMaterials Science & Engineering C-Materials For Biological Applications. Amsterdam, v. 68, p. 338-342, 2016.
dc.identifier0928-4931
dc.identifierhttps://repositorio.unifesp.br/handle/11600/56881
dc.identifier10.1016/j.msec.2016.05.101
dc.identifierWOS:000382600000040
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4028583
dc.description.abstractLayer-by-Layer (LbL) films containing silk fibroin (SF) and the 40 aminoacid-long amyloid-beta peptide (A beta 1-40) were prepared with the purpose of developing a new prototype of an electrochemical immunosensor. The film showed a satisfactory growth in quartz substrate and screen-printed carbon electrodes, as observed by UV-vis spectroscopy and cyclic voltammetric, respectively. The peptide immobilized in LbL films in junction with SF shows secondary structure induced, as shown by circular dichroism measurements, favoring the interaction SF/peptide LbL film with the specific antibody. Immunosensor showed a linear response in the presence of the antibody with concentrations from 0 to 10 ng mL(-1) both analyzed by current changes in 0.3 V and voltammogram area. This system can be applied as a new prototype for preliminary diagnosis of Alzheimer's disease. (C) 2016 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherElsevier Science Bv
dc.relationMaterials Science & Engineering C-Materials For Biological Applications
dc.rightsAcesso restrito
dc.subjectAlzheimer's disease
dc.subjectImmunosensor
dc.subjectSilk fibroin
dc.subjectLayer-by-layer
dc.subjectAmyloid-beta peptide
dc.titleImmunosensor for diagnosis of Alzheimer disease using amyloid-beta 1-40 peptide and silk fibroin thin films
dc.typeArtigo


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