dc.creator | Goncalves, J. M. | |
dc.creator | Lima, L. R. | |
dc.creator | Moraes, Marli Leite de [UNIFESP] | |
dc.creator | Ribeiro, S. J. L. | |
dc.date.accessioned | 2020-07-31T12:47:31Z | |
dc.date.accessioned | 2022-10-07T21:19:39Z | |
dc.date.available | 2020-07-31T12:47:31Z | |
dc.date.available | 2022-10-07T21:19:39Z | |
dc.date.created | 2020-07-31T12:47:31Z | |
dc.date.issued | 2016 | |
dc.identifier | Materials Science & Engineering C-Materials For Biological Applications. Amsterdam, v. 68, p. 338-342, 2016. | |
dc.identifier | 0928-4931 | |
dc.identifier | https://repositorio.unifesp.br/handle/11600/56881 | |
dc.identifier | 10.1016/j.msec.2016.05.101 | |
dc.identifier | WOS:000382600000040 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/4028583 | |
dc.description.abstract | Layer-by-Layer (LbL) films containing silk fibroin (SF) and the 40 aminoacid-long amyloid-beta peptide (A beta 1-40) were prepared with the purpose of developing a new prototype of an electrochemical immunosensor. The film showed a satisfactory growth in quartz substrate and screen-printed carbon electrodes, as observed by UV-vis spectroscopy and cyclic voltammetric, respectively. The peptide immobilized in LbL films in junction with SF shows secondary structure induced, as shown by circular dichroism measurements, favoring the interaction SF/peptide LbL film with the specific antibody. Immunosensor showed a linear response in the presence of the antibody with concentrations from 0 to 10 ng mL(-1) both analyzed by current changes in 0.3 V and voltammogram area. This system can be applied as a new prototype for preliminary diagnosis of Alzheimer's disease. (C) 2016 Elsevier B.V. All rights reserved. | |
dc.language | eng | |
dc.publisher | Elsevier Science Bv | |
dc.relation | Materials Science & Engineering C-Materials For Biological Applications | |
dc.rights | Acesso restrito | |
dc.subject | Alzheimer's disease | |
dc.subject | Immunosensor | |
dc.subject | Silk fibroin | |
dc.subject | Layer-by-layer | |
dc.subject | Amyloid-beta peptide | |
dc.title | Immunosensor for diagnosis of Alzheimer disease using amyloid-beta 1-40 peptide and silk fibroin thin films | |
dc.type | Artigo | |