dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.creatorFeres, Teresa [UNIFESP]
dc.creatorFredianineto, E.
dc.creatorPaiva, Therezinha Bandiera [UNIFESP]
dc.date.accessioned2018-06-15T14:28:09Z
dc.date.accessioned2022-10-07T21:00:35Z
dc.date.available2018-06-15T14:28:09Z
dc.date.available2022-10-07T21:00:35Z
dc.date.created2018-06-15T14:28:09Z
dc.date.issued1994-08-01
dc.identifierBrazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 27, n. 8, p. 1911-1916, 1994.
dc.identifier0100-879X
dc.identifierhttp://repositorio.unifesp.br/11600/43071
dc.identifierWOS:A1994PC60600022
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4025975
dc.description.abstractThe increase in sensitivity of guinea pig preparations to bradykinin (BK) due to stretching occurring with time after mounting was studied by determining the time course of changes in the cell membrane potential, measured with intracellular microelectrodes. A sustained hyperpolarizing effect of BK, which was observed in recently mounted preparations, became transient after 120 min, when it was followed by depolarization, which was much more evident after 4 h of stretching. As a consequence, a parallel increase in the contractile response to BK was also observed. The hyperpolarizing effect was due to the opening of Ca2+-dependent K+ channels sensitive to apamin, since BK dose-response curves done within 1 h of mounting were shifted to the left, becoming similar to dose-response curves obtained 4 h after mounting of the guinea pig ileum preparation. These results were specific for BK, since the potentiating effect of apamin was not observed for acetylcholine. Our results show that the activation of B-2 receptors by BK in the isolated guinea pig ileum induce a dual effect - hyperpolarization and depolarization - and that the increase in the contractile response consequent to stretching is probably due to the inactivation of apamin-sensitive Ca2+-dependent K+ channels.
dc.languageeng
dc.publisherAssoc Bras Divulg Cientifica
dc.relationBrazilian Journal Of Medical And Biological Research
dc.rightsAcesso restrito
dc.subjectBRADYKININ
dc.subjectCA2+-DEPENDENT K+ CHANNEL
dc.subjectAPAMIN
dc.subjectGUINEA PIG ILEUM
dc.subjectSMOOTH MUSCLE STRETCHING
dc.titleMECHANISM OF SMOOTH-MUSCLE CONTRACTION AND RELAXATION MEDIATED BY KININ RECEPTOR
dc.typeArtigo


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