A low-molecular-weight galactofucan from the seaweed, Spatoglossum schroederi, binds fibronectin and inhibits capillary-like tube formation in vitro

dc.creatorMenezes, Maira Maria
dc.creatorNobre, Leonardo Thiago Duarte Barreto [UNIFESP]
dc.creatorRossi, Gustavo Rodrigues
dc.creatorAlmeida-Lima, Jailma
dc.creatorMelo-Silveira, Raniere Fagundes
dc.creatorFranco, Celia Regina Cavichiolo [UNIFESP]
dc.creatorTrindade, Edvaldo da Silva [UNIFESP]
dc.creatorNader, Helena Bonciani [UNIFESP]
dc.creatorRocha, Hugo Alexandre de Oliveira [UNIFESP]
dc.date.accessioned2020-07-20T16:30:57Z
dc.date.accessioned2022-10-07T20:56:29Z
dc.date.available2020-07-20T16:30:57Z
dc.date.available2022-10-07T20:56:29Z
dc.date.created2020-07-20T16:30:57Z
dc.date.issued2018
dc.identifierInternational Journal Of Biological Macromolecules. Amsterdam, v. 111, p. 1067-1075, 2018.
dc.identifier0141-8130
dc.identifierhttps://repositorio.unifesp.br/handle/11600/55608
dc.identifier10.1016/j.ijbiomac.2018.01.119
dc.identifierWOS:000429391000120
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4025110
dc.description.abstractA low-molecular-weight (LMW) heterofucan (designated fucan B) was obtained from the brown seaweed, Spatoglossum schroederi, and its activity as an inhibitor of capillary-like tube formation by endothelial cells (ECs) was analyzed. Chemical, infrared and electrophoretic analyses confirmed the identity of fucan B. In contrast to other LMW fucans, fucan B (0.012-0.1 mg/mL) inhibited ECs capillary-like tube formation in a concentration dependent manner. In addition, fucan B (0.01-0.05 mg/mL) did not affect ECs proliferation. Fucan B also inhibited ECs migration on a fibronectin-coated surface, but not on laminin- or collagen-coated surfaces. Biotinylated fucan B was used as a probe to identify its localization. Confocal microscopy experiments revealed that biotinylated fucan did not bind to the cell surface, but rather only to fibronectin. Our findings suggest that fucan B inhibits ECs capillary-like tube formation and migration by binding directly to fibronectin and blocking fibronectin sites recognized by cell surface ligands. However, further studies are needed to evaluate the in vivo effects of fucan B. (C) 2018 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherElsevier Science Bv
dc.relationInternational Journal Of Biological Macromolecules
dc.rightsAcesso restrito
dc.subjectFucoidan
dc.subjectBrown seaweed, endothelial cells
dc.subjectAnti-angiogenic
dc.titleA low-molecular-weight galactofucan from the seaweed, Spatoglossum schroederi, binds fibronectin and inhibits capillary-like tube formation in vitro
dc.typeArtigo


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