Isolation and possible composition of glucosylceramides from Paracoccidioides brasiliensis

dc.contributorPERKIN ELMER CORP
dc.contributorPACIFIC NW RES FDN
dc.contributorUNIV WASHINGTON
dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.creatorTakahashi, Helio Kiyoshi [UNIFESP]
dc.creatorLevery, S. B.
dc.creatorToledo, Marcos Sergio de [UNIFESP]
dc.creatorSuzuki, E.
dc.creatorSalyan, MEK
dc.creatorHakomori, S.
dc.creatorStraus, Anita Hilda [UNIFESP]
dc.date.accessioned2018-06-15T16:23:58Z
dc.date.accessioned2022-10-07T20:56:20Z
dc.date.available2018-06-15T16:23:58Z
dc.date.available2022-10-07T20:56:20Z
dc.date.created2018-06-15T16:23:58Z
dc.date.issued1996-11-01
dc.identifierBrazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 29, n. 11, p. 1441-1444, 1996.
dc.identifier0100-879X
dc.identifierhttp://repositorio.unifesp.br/11600/43098
dc.identifierWOS:A1996VR61500005
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4025076
dc.description.abstractTwelve different species of neutral monohexosyl ceramides (CMHs) and two species of neutral monohexosyl ceramides were isolated from mycelium and yeast forms of Paracoccidioides brasiliensis, respectively, by a combination of ion-exchange chromatography, HPLC, and HPTLC. The glucosylceramides did not react with sera from patients with paracoccidioidomycosis (PCM). Carbohydrate analysis indicated that CMHs contain glucose. Analysis of H-1-NMR and mass spectrometry data suggest that the structure of the CMHs is Glcp beta 1-->Cer (mycelium forms present 12 different ceramides and yeast forms present 2 different ceramides). The composition of the lipid moieties was analyzed by negative fast atom bombardment mass spectrometry. No glycosphingolipid other than glucosylceramide was detected in P. brasiliensis.
dc.languageeng
dc.publisherAssoc Bras Divulg Cientifica
dc.relationBrazilian Journal Of Medical And Biological Research
dc.rightsAcesso restrito
dc.subjectglycosphingolipids
dc.subjectglucosylceramide
dc.subjectParacoccidioides brasiliensis
dc.subjectfast atom bombardment mass spectrometry
dc.titleIsolation and possible composition of glucosylceramides from Paracoccidioides brasiliensis
dc.typeArtigo


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