Application of Real-Time PCR Stool Assay for Helicobacter pylori Detection and Clarithromycin Susceptibility Testing in Brazilian Children

dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.contributorUniversidade de São Paulo (USP)
dc.creatorScaletsky, Isabel C. A. [UNIFESP]
dc.creatorAranda, Katia R. S. [UNIFESP]
dc.creatorGarcia, Gabriela T. [UNIFESP]
dc.creatorGoncalves, Manoel E. P.
dc.creatorCardoso, Silvia R.
dc.creatorIriya, Kiyoshi
dc.creatorSilva, Neusa P. [UNIFESP]
dc.date.accessioned2016-01-24T14:17:04Z
dc.date.accessioned2022-10-07T20:53:35Z
dc.date.available2016-01-24T14:17:04Z
dc.date.available2022-10-07T20:53:35Z
dc.date.created2016-01-24T14:17:04Z
dc.date.issued2011-08-01
dc.identifierHelicobacter. Malden: Wiley-Blackwell, v. 16, n. 4, p. 311-315, 2011.
dc.identifier1083-4389
dc.identifierhttp://repositorio.unifesp.br/handle/11600/33943
dc.identifier10.1111/j.1523-5378.2011.00845.x
dc.identifierWOS:000292867900008
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4024372
dc.description.abstractBackground: Helicobacter pylori ClariRes assay is a novel commercially available real-time PCR assay allowing H. pylori detection and clarithromycin susceptibility testing in either gastric biopsy or stool specimens.Objective: the aim of this study was to validate the novel biprobe real-time assay in stool specimens from 217 dyspeptic children.Methods: DNA from gastric biopsies and stool specimens were obtained and submitted to the biprobe real time assay for H. pylori detection and clarithromycin susceptibility testing.Results: the sensitivity, specificity, and test accuracy were 69, 100 and 93.9% for the detection of H. pylori infection and 83.3, 100 and 95.6%, for detection of clarithromycin resistance.Conclusion: This assay proved to be appropriate for H. pylori clarithromycin susceptibility testing, particularly in children populations where a high prevalence of clarithromycin-resistant strains is suspected.
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationHelicobacter
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.rightsAcesso restrito
dc.subjectHelicobacter pylori
dc.subjectstool PCR
dc.subjectchildren
dc.titleApplication of Real-Time PCR Stool Assay for Helicobacter pylori Detection and Clarithromycin Susceptibility Testing in Brazilian Children
dc.typeArtigo


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