dc.contributor | Universidade de São Paulo (USP) | |
dc.contributor | Universidade Federal de São Paulo (UNIFESP) | |
dc.creator | Ganiko, L. | |
dc.creator | Martins, A. R. | |
dc.creator | Freymuller, E. | |
dc.creator | Mortara, R. A. | |
dc.creator | Roque-Barreira, M. C. | |
dc.date.accessioned | 2016-01-24T12:37:36Z | |
dc.date.accessioned | 2022-10-07T20:51:42Z | |
dc.date.available | 2016-01-24T12:37:36Z | |
dc.date.available | 2022-10-07T20:51:42Z | |
dc.date.created | 2016-01-24T12:37:36Z | |
dc.date.issued | 2005-01-18 | |
dc.identifier | Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1721, n. 1-3, p. 152-163, 2005. | |
dc.identifier | 0304-4165 | |
dc.identifier | http://repositorio.unifesp.br/handle/11600/28115 | |
dc.identifier | 10.1016/j.bbagen.2004.10.012 | |
dc.identifier | WOS:000226422500018 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/4023978 | |
dc.description.abstract | The lectin KM+ from Artocarpus integrifolia, also known as artocarpin, induces neutrophil migration by haptotaxis. the interactions of KM+ with both the extracellular matrix (ECM) and neutrophils depend on the lectin ability to recognize mannose-containing glycans. Here, we report the binding of KM+ to laminin and demonstrate that this interaction potentiates the KM+-induced neutrophil migration. Labeling of lung tissue by KM+ located its ligands on the endothelial cells, in the basement membrane, in the alveolus, and in the interstitial connective tissue. Such labeling was inhibited by 400 mM D-mannose, 10 mM Manalpha1-3[Manalpha1-6]Man or 10 muM peroxidase (a glycoprotein-containing mannosyl heptasaccharide). Laminin is a tissue ligand for KM+, since both KM+ and anti-laminin antibodies not only reacted with the same high molecular mass components of a lung extract, but also determined colocalized labeling in basement membranes of the lung tissue. the relevance of the KM+-laminin interaction to the KM+ property of inducing neutrophil migration was evaluated. the inability of low concentrations of soluble KM+ to induce human neutrophil migration was reversed by coating the microchamber filter with laminin. So, the interaction of KM+ with laminin promotes the formation of a substrate-bound KM+ gradient that is able to induce neutrophil haptotaxis. (C) 2004 Elsevier B.V. All rights reserved. | |
dc.language | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation | Biochimica Et Biophysica Acta-general Subjects | |
dc.rights | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dc.rights | Acesso restrito | |
dc.subject | lectin | |
dc.subject | Artocarpus integrifolia | |
dc.subject | neutrophil haptotaxis | |
dc.subject | extracellular matrix | |
dc.subject | laminin | |
dc.subject | haptotactic gradient | |
dc.title | Lectin KM+-induced neutrophil haptotaxis involves binding to laminin | |
dc.type | Artigo | |