dc.creator | Silva, Suelen Feitoza | |
dc.creator | Levy, Debora | |
dc.creator | Maria Ruiz, Jorge Luis | |
dc.creator | de Melo, Thatiana Correa | |
dc.creator | Isaac, Cesar | |
dc.creator | Fidelis, Maira Luisa [UNIFESP] | |
dc.creator | Rodrigues, Alessandro [UNIFESP] | |
dc.creator | Bydlowski, Sergio Paulo | |
dc.date.accessioned | 2020-07-13T11:53:24Z | |
dc.date.accessioned | 2022-10-07T20:47:36Z | |
dc.date.available | 2020-07-13T11:53:24Z | |
dc.date.available | 2022-10-07T20:47:36Z | |
dc.date.created | 2020-07-13T11:53:24Z | |
dc.date.issued | 2017 | |
dc.identifier | Journal Of Steroid Biochemistry And Molecular Biology. Oxford, v. 169, p. 164-175, 2017. | |
dc.identifier | 0960-0760 | |
dc.identifier | https://repositorio.unifesp.br/handle/11600/54604 | |
dc.identifier | 10.1016/j.jsbmb.2016.04.017 | |
dc.identifier | WOS:000401391300019 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/4022947 | |
dc.description.abstract | Mesenchymal stem cells (MSCs) are multipotent cells characterized by self-renewal and cellular differentiation capabilities. Oxysterols comprise a very heterogeneous group derived from cholesterol through enzymatic and non-enzymatic oxidation. Potent effects in cell death processes, including cytoxicity and apoptosis induction, were described in several cell lines. Very little is known about the effects of oxysterols in MSCs. 7-ketocholesterol (7-KC), one of the most important oxysterols, was shown to be cytotoxic to human adipose tissue-derived MSCs. Here, we describe the short-term (24 h) cytotoxic effects of cholestan-3 alpha-5 beta-6 alpha-triol, 3,5 cholestan-7-one, (3 alpha-5 beta-6 alpha)- cholestane-3,6-diol, 7-oxocholest-5-en-3 beta-ylacetate, and 5 beta-6 beta epoxy-cholesterol, on MSCs derived from human adipose tissue. MSCs were isolated from adipose tissue obtained from three young, healthy women. Oxysterols, with the exception of 3,5 cholestan-7-one and 7-oxocholest-5-en-3 beta-yl acetate, led to a complex mode of cell death that include apoptosis, necrosis and autophagy, depending on the type of oxysterol and concentration, being cholestan-3 alpha-5 beta-6 alpha-triol the most effective. Inhibition of proliferation was also promoted by these oxysterols, but no changes in cell cycle were observed. (C) 2016 Elsevier Ltd. All rights reserved. | |
dc.language | eng | |
dc.publisher | Pergamon-Elsevier Science Ltd | |
dc.relation | Journal Of Steroid Biochemistry And Molecular Biology | |
dc.rights | Acesso restrito | |
dc.subject | Oxysterol | |
dc.subject | Mesenchymal stem cell | |
dc.subject | Apoptosis | |
dc.subject | Cell death | |
dc.subject | Mitochondrial hyperpolarization | |
dc.subject | Adipose tissue | |
dc.title | Oxysterols in adipose tissue-derived mesenchymal stem cell proliferation and death | |
dc.type | Artigo | |