Synthesis and pharmacological properties of TOAC-labeled angiotensin and bradykinin analogs

dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.contributorUNESP
dc.contributorUniversidade de São Paulo (USP)
dc.creatorNakaie, Clovis Ryuichi [UNIFESP]
dc.creatorSilva, Eneida de Gusmão [UNIFESP]
dc.creatorCilli, Eduardo Maffud [UNIFESP]
dc.creatorMarchetto, Reinaldo [UNIFESP]
dc.creatorSchreier, Shirley [UNIFESP]
dc.creatorPaiva, Therezinha Bandiera [UNIFESP]
dc.creatorPaiva, Antonio Cechelli de Mattos [UNIFESP]
dc.date.accessioned2016-01-24T12:33:11Z
dc.date.accessioned2022-10-07T20:47:33Z
dc.date.available2016-01-24T12:33:11Z
dc.date.available2022-10-07T20:47:33Z
dc.date.created2016-01-24T12:33:11Z
dc.date.issued2002-01-01
dc.identifierPeptides. New York: Elsevier B.V., v. 23, n. 1, p. 65-70, 2002.
dc.identifier0196-9781
dc.identifierhttp://repositorio.unifesp.br/handle/11600/26703
dc.identifier10.1016/S0196-9781(01)00580-0
dc.identifierWOS:000173678100009
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4022937
dc.description.abstractAngiotensin II (AngII) and bradykinin (BK) derivatives containing the TOAC (2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid) spin label were synthesized by solid phase methodology. Ammonium hydroxide (pH 10, 50degreesC, 1 h) was the best means for reverting nitroxide protonation occurring during peptide cleavage. EPR spectra yielded rotational correlation times for internally labeled analogs that were nearly twice as large as those of N-terminally labeled analogs. Except for TOAC(1)-AngII and TOAC(0)-BK, which showed high intrinsic activities, other derivatives were inactive in smooth muscle preparations. These active paramagnetic analogs may be useful for conformational studies in solution and in the presence of model and biological membranes. (C) 2002 Elsevier Science Inc. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationPeptides
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.rightsAcesso restrito
dc.subjectangiotensin analogs
dc.subjectbradykinin analogs
dc.subjectpeptide synthesis
dc.subjectspin labeled peptides
dc.subjectTOAC spin label
dc.subjectelectron paramagnetic resonance
dc.titleSynthesis and pharmacological properties of TOAC-labeled angiotensin and bradykinin analogs
dc.typeArtigo


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