dc.creator | Morais, Katia Luciano Pereira [UNIFESP] | |
dc.creator | Fernandes Pacheco, Mario Thiego | |
dc.creator | Berra, Carolina Maria | |
dc.creator | Bosch, Rosemary V. | |
dc.creator | Sciani, Juliana Mozer | |
dc.creator | Chammas, Roger [UNIFESP] | |
dc.creator | Saito, Renata de Freitas | |
dc.creator | Iqbal, Asif | |
dc.creator | Chudzinski-Tavassi, Ana Marisa [UNIFESP] | |
dc.date.accessioned | 2020-07-22T13:23:17Z | |
dc.date.accessioned | 2022-10-07T20:46:53Z | |
dc.date.available | 2020-07-22T13:23:17Z | |
dc.date.available | 2022-10-07T20:46:53Z | |
dc.date.created | 2020-07-22T13:23:17Z | |
dc.date.issued | 2016 | |
dc.identifier | Molecular And Cellular Biochemistry. Dordrecht, v. 415, p. 119-131, 2016. | |
dc.identifier | 0300-8177 | |
dc.identifier | https://repositorio.unifesp.br/handle/11600/56153 | |
dc.identifier | WOS000373610000011.pdf | |
dc.identifier | 10.1007/s11010-016-2683-4 | |
dc.identifier | WOS:000373610000011 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/4022759 | |
dc.description.abstract | During the last two decades, new insights into proteasome function and its role in several human diseases made it a potential therapeutic target. In this context, Amblyomin-X is a Kunitz-type FXa inhibitor similar to endogenous tissue factor pathway inhibitor (TFPI) and is a novel proteasome inhibitor. Herein, we have demonstrated Amblyomin-X cytotoxicity to different tumor cells lines such as pancreatic (Panc1, AsPC1BxPC3) and melanoma (SK-MEL-5 and SK-MEL-28). Of note, Amblyomin-X was not cytotoxic to normal human fibroblast cells. In addition, Amblyomin-X promoted accumulation of ER stress markers (GRP78 and GADD153) in sensitive (SK-MEL-28) and bortezomib-resistant (Mia-PaCa-2) tumor cells. The intracellular calcium concentration [Ca2+] (i) was slightly modulated in human tumor cells (SK-MEL-28 and Mia-PaCa-2) after 24 h of Amblyomin-X treatment. Furthermore, Amblyomin-X induced mitochondrial dysfunction, cytochrome-c release, PARP cleavage, and activation of caspase cascade in both human tumor (SK-MEL-28 and Mia-PaCa-2) cells. These investigations might help in further understanding of the antitumor properties of Amblyomin-X. | |
dc.language | eng | |
dc.publisher | Springer | |
dc.relation | Molecular And Cellular Biochemistry | |
dc.rights | Acesso aberto | |
dc.subject | Amblyomin-X | |
dc.subject | Kunitz-type inhibitor | |
dc.subject | Proteasome inhibitor | |
dc.subject | Endoplasmic reticulum stress | |
dc.subject | Antitumor drug candidate | |
dc.title | Amblyomin-X induces ER stress, mitochondrial dysfunction, and caspase activation in human melanoma and pancreatic tumor cell | |
dc.type | Artigo | |