dc.contributor | Universidade Federal de São Paulo (UNIFESP) | |
dc.contributor | BIOMERIEUX | |
dc.creator | Franco, Fulvia Regina de Souza [UNIFESP] | |
dc.creator | Paranhos-Baccala, Glaucia S. | |
dc.creator | Yamauchi, Lucy Megumi [UNIFESP] | |
dc.creator | Yoshida, Nobuko [UNIFESP] | |
dc.creator | Silveira, Jose Franco da [UNIFESP] | |
dc.date.accessioned | 2018-06-15T17:38:36Z | |
dc.date.accessioned | 2022-10-07T20:40:23Z | |
dc.date.available | 2018-06-15T17:38:36Z | |
dc.date.available | 2022-10-07T20:40:23Z | |
dc.date.created | 2018-06-15T17:38:36Z | |
dc.date.issued | 1993-10-01 | |
dc.identifier | Infection And Immunity. Washington: Amer Soc Microbiology, v. 61, n. 10, p. 4196-4201, 1993. | |
dc.identifier | 0019-9567 | |
dc.identifier | http://repositorio.unifesp.br/11600/43949 | |
dc.identifier | WOS:A1993LZ26400026 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/4020967 | |
dc.description.abstract | We have cloned and sequenced a cDNA for a metacyclic trypomastigote-specific glycoprotein, with a molecular mass of 90 kDa, termed MTS-gp90. By immunoblotting, antibodies to the MTS-gp90 recombinant protein reacted exclusively with a 90-kDa antigen of metacyclic trypomastigotes. The insert of the MTS-gp90 cDNA clone strongly hybridized with a single 3.0-kb mRNA of metacyclic forms, whereas the hybridization signal with epimastigote mRNA was weak and those with RNAs from other developmental stages were negative, indicating that transcription of the MTS-gp90 gene is developmentally regulated. A series of experiments showed that the MTS-gp90 gene is present in multiple copies in the Trypanosoma cruzi genome, arranged in a nontandem manner, and that there are at least 40 copies of the gene per haploid genome. Sequence analysis of recombinant MTS-gp90 revealed 40 to 60% identity at the amino acid level with members of a family of mammalian stage-specific, 85-kDa surface antigens of T. cruzi. However, there are considerable differences in the amino acid compositions outside the homology region. | |
dc.language | eng | |
dc.publisher | Amer Soc Microbiology | |
dc.relation | Infection And Immunity | |
dc.rights | Acesso restrito | |
dc.title | CHARACTERIZATION OF A CDNA CLONE ENCODING THE CARBOXY-TERMINAL DOMAIN OF A 90-KILODALTON SURFACE-ANTIGEN OF TRYPANOSOMA-CRUZI METACYCLIC TRYPOMASTIGOTES | |
dc.type | Artigo | |