Immunolocalization of Leishmania (Viannia) braziliensis membrane antigens recognized by mAbs SST-2, SST-3, and SST-4

dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.creatorSilveira, TGV
dc.creatorTakahashi, H. K.
dc.creatorStraus, A. H.
dc.date.accessioned2016-01-24T12:34:07Z
dc.date.accessioned2022-10-07T20:34:03Z
dc.date.available2016-01-24T12:34:07Z
dc.date.available2022-10-07T20:34:03Z
dc.date.created2016-01-24T12:34:07Z
dc.date.issued2003-11-01
dc.identifierParasitology. New York: Cambridge Univ Press, v. 127, p. 449-456, 2003.
dc.identifier0031-1820
dc.identifierhttp://repositorio.unifesp.br/handle/11600/27471
dc.identifier10.1017/S0031182003003986
dc.identifierWOS:000187030600005
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4019525
dc.description.abstractThe immunolocalization of Leishmania (Viannia) braziliensis stage-specific antigens recognized by mAbs was analysed by transmission electron microscopy. the antigen recognized by mAb SST-2 was present at the surface of promastigotes, including the flagellum and flagellar pocket. the reactivity of SST-2 with isolates of different serodemes showed a pronounced microheterogencity in terms of the number of reactive bands within the low molecular weight range from 24 to 33 kDa. the 180 kDa glycoprotein recognized by mAb SST-3 was present only in the flagellar membrane. SST-3 also recognized multiple discrete bands from 160 to 200 kDa, as observed in several serodemes. in contrast, mAb SST-4, which recognizes a 98 kDa antigen, showed weak labelling on the promastigote surface by transmission electron microscopy and indirect immunofluorescence. Based on Western blotting, indirect immunofluorescence, and solid-phase radioimmunoassay, the antigens recognized by mAbs SST-2, SST-3 and SST-4 were present in all L. (V.) braziliensis analysed, from 7 different serodemes.
dc.languageeng
dc.publisherCambridge Univ Press
dc.relationParasitology
dc.rightshttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676
dc.rightsAcesso restrito
dc.subjectLeishmania (Viannia) braziliensis
dc.subjectmonoclonal antibody
dc.subjectspecific antigens
dc.subjectflagellum
dc.titleImmunolocalization of Leishmania (Viannia) braziliensis membrane antigens recognized by mAbs SST-2, SST-3, and SST-4
dc.typeArtigo


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