dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.contributorDent Sch Ribeirao Preto
dc.creatorSteiner, A. A.
dc.creatorAntunes-Rodrigues, J.
dc.creatorBranco, LGS
dc.date.accessioned2016-01-24T12:33:28Z
dc.date.accessioned2022-10-07T20:30:48Z
dc.date.available2016-01-24T12:33:28Z
dc.date.available2022-10-07T20:30:48Z
dc.date.created2016-01-24T12:33:28Z
dc.date.issued2002-07-19
dc.identifierBrain Research. Amsterdam: Elsevier B.V., v. 944, n. 1-2, p. 135-145, 2002.
dc.identifier0006-8993
dc.identifierhttp://repositorio.unifesp.br/handle/11600/26932
dc.identifier10.1016/S0006-8993(02)02738-5
dc.identifierWOS:000177224100014
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4018926
dc.description.abstractThe present study aimed to test the hypothesis that a decrease in preoptic cAMP mediates fever. To this end, body core temperature (T-c) of unanesthetized, freely moving rats was monitored by biotelemetry before and after pharmacological modulation of the cAMP pathway, and cAMP levels in the anteroventral third ventricular region (AV3V), where the preoptic region (POA) is located, were determined. We observed that intra-POA administration of the cAMP agonist dibutyryl-cAMP (Db-cAMP, 40 mug) reduced T-c. PGE(2) (the proximal mediator of fever, 200 ng) raised T-c with a concomitant decrease in AV3V cAMP levels from 22.7+/-1.8 to 17.0+/-1.0 fmol/mug protein. Moreover, PGE(2)-induced fever was impaired by the phosphodiesterase inhibitor aminophylline. in order to verify the interaction between the cAMP- and cGMP-dependent pathways in the POA, we then co-injected Db-cAMP and 8-Br-cGMP into the POA. As a result, 8-Br-cGMP augmented the drop in 7 evoked by Db-cAMP. Lastly, we observed that intra-POA co-microinjection of the protein kinase A inhibitor (Rp-cAMPS, 1 mug) with the protein kinase G inhibitor (Rp-cGMPS, 1 mug), mimicking the effects of reduced production of cAMP and cGMP, respectively, produced a fever-like response. in summary, the present data support that a decrease in the levels of cAMP and cGMP in the POA is associated with the genesis of fever. (C) 2002 Elsevier Science BY. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationBrain Research
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.rightsAcesso restrito
dc.subjectbody temperature
dc.subjectprostaglandin
dc.subjectnitric oxide
dc.subjectset point
dc.subjecthypothalamus
dc.subjecttemperature-sensitive neuron
dc.subjectcyclic AMP
dc.subjectcyclic GMP
dc.titleRole of preoptic second messenger systems (cAMP and cGMP) in the febrile response
dc.typeArtigo


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