Artigo de Periódico
A simple and sensitive UFLC-fluorescence method for endocrine disrupters determination in marine waters
Fecha
2013Registro en:
0039-9140
v. 117
Autor
Lisboa, Normando S.
Fahning, Cristiane S.
Cotrim, Gabriel
Anjos, Jeancarlo Pereira dos
Andrade, Jailson Bittencourt de
Hatje, Vanessa
Rocha, Gisele Olímpio da
Lisboa, Normando S.
Fahning, Cristiane S.
Cotrim, Gabriel
Anjos, Jeancarlo Pereira dos
Andrade, Jailson Bittencourt de
Hatje, Vanessa
Rocha, Gisele Olímpio da
Institución
Resumen
The present study proposes a fast and simple analytical methodology employing C18 SPE cartridges (for preconcentration and clean-up), and a ultra-fast liquid chromatography coupled to fluorescence detector (UFLC-FLD) for determination of the following endocrine disrupters (ED): bisphenol A (BPA), 4-n-nonylphenol (4NNP), 4-n-octylphenol (4NOP), 4-t-octylphenol (4TOP), estriol (E3), estrone (E1), 17β-estradiol (E2) and 17α-ethynylestradiol (EE2) in seawater. The proposed method was developed, optimized and validated. Separation was done by a total running time of 10 min in a Shim-pack XR-ODS C-18 (2.0 mm ID × 50 mm) chromatographic column, mobile phases were acetonitrile/ultra-pure water under gradient programming; eluent flow rate at 0.120 mL min−1; column temperature set at 60 °C; emission wavelength of 306 nm and excitation wavelength of 280 nm. The method was validated through assessment of the following parameters: linear range, linearity, selectiveness, precision, recovery test, limit of detection (LOD), and limit of quantification (LOQ). Recoveries ranged from 91% (for EE2) to 104% (for 4NNP) and also was found a suitable repeatability (RSD <4.5%) for all considered compounds. LOD and LOQ ranged from 2.0 ng L−1 (EE2) to 23 ng L−1 (E1) and 9.3 ng L−1 (EE2) to 96 ng L−1 (E1), respectively. The analytical method using SPE UFLC-FLD was applied to seawater samples collected from Todos os Santos Bay (BTS), Brazil to determine the concentration of eight ED.