Artigo de Periódico
Downregulation of IFN-γ production in patients with recurrent vaginal candidiasis
Fecha
2002-01Registro en:
0091-6749
v. 109, n 1
Autor
Carvalho, Lucas Pedreira de
Bacellar, Maria Olívia Amado Ramos
Neves, Nilma Antas
Jesus, Amélia Maria Ribeiro de
Carvalho Filho, Edgar Marcelino de
Carvalho, Lucas Pedreira de
Bacellar, Maria Olívia Amado Ramos
Neves, Nilma Antas
Jesus, Amélia Maria Ribeiro de
Carvalho Filho, Edgar Marcelino de
Institución
Resumen
Background: Recurrent vaginal candidiasis (RVC) is an important health problem with unknown pathogenesis. Although impairment of the T-cell response is associated with persistent or recurrent candidiasis, data on immunologic responses in patients with RVC are controversial. Objectives: To evaluate the T-cell response in patients with RVC and the ability of cytokines and cytokine antagonists to modulate IFN-γ production in cultures stimulated with Candida albicans antigens. Methods: Participants in the study included 13 patients with RVC and 7 control women with sporadic candidiasis. Cytokines were determined by ELISA in supernatants of mononuclear cells with C albicans , purified protein derivative, or tetanus toxoid antigen. Results: IFN-γ production was absent or low in 11 of 13 women (84.6%) with RVC. Absent or low IFN-γ production was specific to C albicans antigens (189 ± 389 pg/mL), because high IFN-γ levels were found in cultures stimulated with purified protein derivative (739 ± 774 pg/mL) or tetanus toxoid antigens (1085 ± 546 pg/mL). Monoclonal antibody anti-IL-10 enhanced IFN-γ levels (750 ± 753 pg/mL), and IL-10 suppressed this cytokine production in patients with sporadic candidiasis. Conclusions: Mononuclear cells from patients with RVC stimulated with C albicans antigen have low or absent IFN-γ production. IL-10 plays an important role in downregulation of the T-cell response in these patients. (J Allergy Clin Immunol 2002;109:102-5.)