Expressão imuno-histoquímica de colágeno IV, Tenascina-c e Fibronectina em lesões centrais e periféricas de células gigantes

Abstract

Introdution – The central and peripheral giant cells lesions represent a group of pathological entities that despite exhibiting similar histopatological features, etiology and nature are not entirely clear. Methods – It was performed an immunohistochemical analysis of 8 cases of peripheral giant cell lesion (PGCLs) and 16 cases of central giant cell lesion (CGCLs) obtained from the archives of Surgical Oral Pathology of Federal University of Rio Grande do Norte, in relation to expression and distribution of extracellular matrix proteins collagen IV, tenascin-C and fibronectin. Results – The specimens revealed discontinuous expression of collagen IV in epithelial basement membrane of PGCLs, commonly associated with areas showing inflammatory cells. Additionally, collagen IV was observed in vascular basal membrane of PGCLs and CGCLs, showing continuous pattern, fading from periphery to center areas. Tenascin-C expression was verified in extracellular matrix displaying reticular and fibrillar patterns and predominantly diffuse and heterogeneous distribution both in PGCLs and CGCLs. Immunohistochemical expression of fibronectin was observed equally in PGCLs and CGCLs, exhibiting a uniform distribution through extracellular matrix, with reticular and fibrillar patterns, mainly in the neighborhood of mononucleated and multinucleated cells. Conclusion – The results obtained demonstrated no significant differences in the pattern of expression of collagen IV, tenascin-C and fibronectin among PGCLs and CGCLs studied.