dc.contributorXimenes, Maria de Fátima Freire de Melo
dc.contributor
dc.contributorhttp://lattes.cnpq.br/6945268593644384
dc.contributor
dc.contributorhttp://lattes.cnpq.br/4231925509338101
dc.contributorUchoa, Adriana Ferreira
dc.contributor
dc.contributorhttp://lattes.cnpq.br/6644671747055211
dc.contributorMedeiros, Caroline Addison Carvalho Xavier de
dc.contributor
dc.contributorhttp://lattes.cnpq.br/2982271986555450
dc.contributorRocha, Hugo Alexandre De Oliveira
dc.contributor
dc.contributorhttp://lattes.cnpq.br/4651814546820796
dc.contributorDantas, Iron Macedo
dc.contributor
dc.contributorhttp://lattes.cnpq.br/6446119209274157
dc.contributorPaiva, Patrícia Maria Guedes
dc.contributor
dc.contributorhttp://lattes.cnpq.br/2885145995086459
dc.creatorBarbosa, Patricia Batista Barra Medeiros
dc.date.accessioned2016-01-25T12:17:39Z
dc.date.accessioned2022-10-06T13:55:06Z
dc.date.available2016-01-25T12:17:39Z
dc.date.available2022-10-06T13:55:06Z
dc.date.created2016-01-25T12:17:39Z
dc.date.issued2014-06-09
dc.identifierBARBOSA, Patricia Batista Barra Medeiros. Atividades biológicas de extratos salinos de sementes de plantas da caatinga contra Aedes Aegypti e investigação da participação de proteínas bioativas. 2014. 135f. Tese (Doutorado em Bioquímica) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2014.
dc.identifierhttps://repositorio.ufrn.br/jspui/handle/123456789/19653
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3973678
dc.description.abstractDengue fever, currently the most important arbovirus, is transmitted by the bite of the Aedes aegypti mosquito. Given the absence of a prophylactic vaccine, the disease can only be controlled by combating the vector insect. However, increasing reports of resistance and environmental damage caused by insecticides have led to the urgent search for new safer alternatives. Twenty - um plant s eed extracts from the Caatinga were prepared , tested and characterized . Sodium phosphate ( 50 mM pH 8.0) was used as extractor. All extracts showed larvicidal and ovipositional deterrence activity . Extracts of D. grandiflora, E. contortisiliquum, A. cearenses , C. ferrea and C. retusa were able to attract females for posture when in low co ncentration . In the attractive concentrations, the CE of E. contortisiliquum and A. cearenses were able to kill 52% and 100% of the larvae respectively . The extracts of A. cearenses , P. viridiflora, E. velutina, M. urundeuva and S. brasiliensis were also pupicides, while extracts of P. viridiflora, E. velutina, E. contortisiliquum , A. cearenses, A. colubrina, D. grandiflora , B. cheilantha , S. spectabilis, C. pyramidalis, M. regnelli e G. americana displayed adulticidal activity. All extracts were toxic to C. dubia zooplankton . The EB of E. velutina and E. contortisiliquum did not affect the viability of fibroblasts . In all extracts were identified at least two potential insecticidal proteins such as enzyme inhibitors, lectins and chitin - binding proteins and components of secondary metabolism . Considering all bioassays , the extracts from A. cearenses, P. viridiflora, E. contortisiliquum , S. brasiliensis, E. velutina and M. urundeuva were considered the most promising . The E. contortisiliquum extracts was the only one who did not show pupicida activity, indicating that its mechanism of action larvicide and adulticidal is related only to the ingesti on of toxic compounds by insect , so it was selected to be fragmenting. As observed for the CE , th e protein fractions of E. contortisiliquum also showed larvicidal activity, highlighting that F2 showed higher larvicidal activity and lower en vironmental toxicity than the CE source. The reduction in the proteolytic activity of larvae fed with crude extra ct and fractions of E. contortisiliquum suggest ed that the trypsin inhibitors ( ITEc) would be resp onsible for larvicidal activity . However the increase in the purification of this inhibitor resulted in loss of larvicidal activity , but the absence of trypsin inhibitor reduced the effectiveness of the fractions , indicating that the ITEC contributes to the larvicidal activity of this extract. Not been observed larvicidal activity and adulticide in rich fraction vicilin, nor evidence of the contribution o f this molecule for the larvicidal activity of the extract. The results show the potential of seeds from plant extracts of Caatinga as a source of active molecules against insects A. aegypti at different stages of its development cycle, since they are comp osed of different active compounds, including protein nature, which act on different mechanisms should result in the death of insec
dc.languagepor
dc.publisherUniversidade Federal do Rio Grande do Norte
dc.publisherBrasil
dc.publisherUFRN
dc.publisherPROGRAMA DE PÓS-GRADUAÇÃO EM BIOQUÍMICA
dc.rightsAcesso Aberto
dc.subjectLarvicida
dc.subjectPupicida
dc.subjectAdulticida
dc.subjectIndice de repelência efetiva
dc.subjectHomogenato intestinal
dc.subjectInibidores enzimáticos
dc.subjectLectinas
dc.subjectVicilinas
dc.titleAtividades biológicas de extratos salinos de sementes de plantas da caatinga contra Aedes Aegypti e investigação da participação de proteínas bioativas
dc.typedoctoralThesis


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