masterThesis
Caracterização funcional de dois cDNAs de cana-de- açúcar : PKCI e SHAGGY
Fecha
2011-05-16Registro en:
SILVA, Francinaldo Leite da. Caracterização funcional de dois cDNAs de cana-de- açúcar : PKCI e SHAGGY. 2011. 90 f. Dissertação (Mestrado em Biodiversidade; Biologia Estrutural e Funcional.) - Universidade Federal do Rio Grande do Norte, Natal, 2011.
Autor
Silva, Francinaldo Leite da
Resumen
Flowering is controlled by several environmental and endogenous factors, usually associated with a complex network of metabolic mechanisms. The gene characterization in Arabidopsis model has provided much information about the genetic and molecular mechanisms that control flowering process. Some of these genes had been found in rice and maize. However, in sugarcane this
processe is not well known. It is known that early flowering may reduce its production up to 60% at northeast conditions. Considering the impact of early flowering in sugarcane production, the aim of this work was to make the gene
characterization of two cDNAs previously identified in subtractive cDNA libraries: scPKCI and scSHAGGY. The in silico analysis showed that these two cDNAs presented both their sequence and functional catalytic domains conserved. The results of transgenic plants containing the overexpression of the
gene cassette scPKCI in sense orientation showed that this construction had a negative influence on the plant development as it was observed a decrease in plant height and leaf size. For the scPKCI overexpression in antisense orientation it was observed change in the number of branches from T1
transgenic plants, whereas transgenic T2 plants showed slow development during germination and initial stages of development. The other cDNA analyzed had homology to SHAGGY protein. The overexpression construct in sense
orientation did not shown any effect on development. The only difference observed it was an increase in stigma structure. These results allowed us to propose a model how these two genes may be interact and affect floweringdevelopment.