masterThesis
Análise imuno-histoquímica das proteínas SHH, SMO E GLI-1 em lesões benignas do epitélio odontogênico
Fecha
2019-10-24Registro en:
RODRIGUES, Katianne Soares. Análise imuno-histoquímica das proteínas SHH, SMO E GLI-1 em lesões benignas do epitélio odontogênico. 2019. 84f. Dissertação (Mestrado em Ciências Odontológicas) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2019.
Autor
Rodrigues, Katianne Soares
Resumen
INTRODUCTION: The odontogenic cysts and tumors represent heterogeneous groups of
lesions that affect the gnatic bones and which have not yet fully clarified pathogenesis.
Therefore, several studies have been developed in order to analyze if deregulation in some
signaling pathways would be related to the development and progression of these lesions.
Among the studied pathways, we highlight the Sonic Hedgehog pathway, which plays an
important role in the embryonic development of the dental organ and is mutated in some
cancers. AIM: analyze and compare the immunohistochemical expression of proteins involved
in the Sonic Hedgehog signaling pathway (SHH, SMO and GLI-1) in odontogenic keratocysts
(OK), ameloblastomas (AMB) and adenomatoid odontogenic tumors (AOT). MATERIALS
AND METHODS: Protein immunoexpression was semi-quantitatively evaluated in each case
studied and the data were subjected to statistical analysis using the Kruskal-Wallis (KW),
Mann-Whitney (U) and Spearman (r) tests, with the significance level set at 5% (p <0.05).
RESULTS: When analyzing SHH protein in the three lesions, it was observed that AMB
showed significantly higher membrane/ cytoplasmic expression compared to AOT (p = 0,022)
and OK (p = 0,022). Regarding the membrane/cytoplasmic analysis of the SMO, no differences
were identified between the lesions studied. For GLI-1 protein, statistically significant
differences were found at nuclear level for AMB and OK compared to AOT (p < 0,0001). In
addition, positive correlations with statistical significance were observed between cytoplasmic
GLI-1 and nuclear GLI-1 for AMB (r = 0,482; p = 0,031) and OK (r = 0,865; p < 0,0001), and
between membrane/cytoplasmic SMO and cytoplasmic GLI-1 for AOT (r = 0,667; p = 0,035)
and OK (r = 0,535; p = 0,015). CONCLUSIONS: The results of the present study confirm the
participation of this signaling pathway in the pathogenesis of the lesions studied and the
overexpression of SHH in AMBs and nuclear GLI-1 in AMBs and OKs, indicating that these
proteins contribute to the more aggressive biological behavior of these two lesions when
compared to AOT.