masterThesis
Influência do hipoestrogenismo por ooforectomia na adaptação antioxidante em ratas wistar exercitadas regularmente
Fecha
2009-03-31Registro en:
MARCEDO, Ulisvaldo Brunno de Oliveira. Influência do hipoestrogenismo por ooforectomia na adaptação antioxidante em ratas wistar exercitadas regularmente. 2009. 82 f. Dissertação (Mestrado em Bioanálises e Medicamentos) - Universidade Federal do Rio Grande do Norte, Natal, 2009.
Autor
Marcedo, Ulisvaldo Brunno de Oliveira
Resumen
Post-menopause is a period of women s life cycle that is characterized by estrogen
depletion and therefore increasing cardiovascular diseases, neurodegenerative
disorders, urogenital atrophy, osteoporosis, hot flushes and sexual discomfort
incidences. Estrogen is a hormone with comfirmed antioxidant action and its
depletion is related to oxidative stress instalation and damaging various important
biomolecules. Regular physical activity has been identified as a factor involved in
reducing women s post-menopausal complications in addition to improving
antioxidant defense by reducing the oxidative damage and consequently improving
life s quality in this part of the population. This study aims to evaluate the influence of
hypoestrogenism in antioxidant adaptation due to regular exercise, by determining
reduced glutathione (GSH) and Thiobarbituric Acid Reactive Substances (SRAT)
concentrations and antioxidant enzymes glutathione peroxidase (GPx), Superoxide
Dismutase (SOD) and Catalase (CAT) activities in blood, brain and liver of rats. To
achieve this goal we used 50 Wistar rats, weighing 180-250g which were divided into
two groups, control - GC (25) and ooforectomized - GO (25). Each group was
subdivided into five subgroups: Not-trained - S (5), Not-trained Acute Exercise - SEA
(5), regular exercise 30 days - E30 (5), regular exercise 60 days - E60 (5) and regular
exercise 90 days - E90 (5). Each of the three subgroups exercised regularly was
subjected to acute exercise on the eve and the day of sacrifice to collect biological
samples of blood, liver and brain and subsequent determination of SRAT
concentration, GSH content and antioxidant enzymes GPx, SOD and CAT activities.
The results indicated that the sedentary animals acutely exercised presented
oxidative stress and regular physical activity led to antioxidant adaptation. In
ooforectomized group the antioxidant adaptation seen in control animals showed to
be impaired. Unlike the results from blood and liver, in brain there was a shield
against oxidative damage originated by the exercise and that hypoestrogenism led to
a loss of this natural antioxidant potential. Therefore, hypoestrogenism interferes
negatively in antioxidant adaptation due to regular exercise