masterThesis
Estudo de polimorfismos nos genes IL17A e IL17RA e da produção de IL-17A, IL-33 e CCL2 em gestantes infectadas pelo Toxoplasma gondii do Estado do Rio Grande do Norte, Brasil
Fecha
2018-08-31Registro en:
ANDRADE, Joelma Maria de Araújo. Estudo de polimorfismos nos genes IL17A e IL17RA e da produção de IL-17A, IL-33 e CCL2 em gestantes infectadas pelo Toxoplasma gondii do Estado do Rio Grande do Norte, Brasil. 2018. 89f. Dissertação (Mestrado em Ciências Biológicas) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2018.
Autor
Andrade, Joelma Maria de Araújo
Resumen
Toxoplasma gondii (T. gondii) is a parasitic infectious parasitic disease that is an
obligate intracellular parasite capable of determining a delicate balance between
parasitism and the immune response of the host, which is modified due to
environmental and genetic factors. Due to this, we aimed to investigate the allelic and
genotype frequencies of specific SNPs in the IL17A and IL17RA genes, as well as the
production of IL-17A, IL-33, and CCL2 in pregnant women infected with T. gondii from
the State of Rio Grande do Norte, Brazil. Through PCR-RFLP techniques, two SNPs
implicated in Th17 immune response, IL17A rs2275913 (G> A) and IL17RA rs4819554
(A> G) modulation were evaluated in pregnant women, infected or not by T. gondii and
treated in Maternity School Januário Cicco, Natal / RN. These women were also
evaluated for the production of cytokines involved in the modulation of the immune
response, CCL2, IL-33, and IL-17A, by the direct ELISA method. The results showed
that the G-allele of the SNP rs2275913 (IL17A) appeared to be protective in this
population, while the rs4819554 (IL17RA) SNP G allele was associated with a greater
susceptibility to T. gondii infection [P value = 0.025; OR = 2.815 (1.118-7.089); CI =
95%]. Regarding cytokines, none of them had any influence on the analyzed
parameters (abortion and hypertension). In view of these results, this unpublished
study brings important immunogenic evidence in humans that support the existence of
a contribution of the host genotype to the immunomodulation result of T. gondii; this
still opens perspectives for other future approaches of the parasite-host relationship.