bachelorThesis
Estudo piloto: indução da Síndrome dos Ovários Policísticos em camundongos Swiss
Fecha
2019-11-06Registro en:
OLIVEIRA, Annamairlla do Nascimento. Estudo piloto: indução da Síndrome dos Ovários policísticos em camundongos Swiss. Trabalho de conclusão de curso (Graduação em Farmácia) - Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2019.
Autor
Oliveira, Annamairlla do Nascimento
Resumen
Objective: To induce polycystic ovary syndrome (PCOS) in Swiss mice.
Methodology: Examestane was administered through gavage procedure in order to induce PCOS. Eighteen mice were used, these were divided into three groups; control, test I and II. The groups Test I and II received, once a day for 21 days, Examestane at concentrations of 1 and 5 mg /kg p.o, respectively, which was previously dissolved in propyleneglycol at 10% (0.5 mL / kg). The control group received 10% propyleneglycol (0.5 mL / kg) over the same period. Vaginal swabs were collected daily in order to observe the hormonal cytology of each specimen, as well as their weight. At the end of the experiment, the biochemical profile was evaluated from blood serum, which was collected after euthanasia. The ovaries and uterus of each specimen were collected, weighed, evaluated for their anatomy and sent to a histopathological evaluation. Statistical analysis was performed using ANOVA, with Tukey's post-test, for analysis of animal, organ and biochemical data.
Results: A statistically significant variation in right ovary weight was observed comparing the control and test groups. There was no statistical difference in biochemical pattern and animal weight. Although histopathology did not verify the presence of cysts in the ovaries, it observed the presence of leukocyte infiltrate in the ovarian and uterine structures. Cytological evaluation confirmed estrous cycle variation in teste groups.
Conclusion: Given the analysis of the data obtained it is possible to state that there was no development of PCOS, however, the variations presented between the test and control groups indicate that the time and/or dose were insufficient to obtain the expected result.