masterThesis
Imunoexpressão de CLIC4 e α-SMA em carcinoma de células escamosas oral e carcinoma verrucoso oral
Fecha
2018-02-26Registro en:
XEREZ, Mariana Carvalho. Imunoexpressão de CLIC4 e α-SMA em carcinoma de células escamosas oral e carcinoma verrucoso oral. 2018. 96f. Dissertação (Mestrado em Patologia Oral) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2018.
Autor
Xerez, Mariana Carvalho
Resumen
Among the malignant neoplasias affecting the oral cavity, oral squamous cell carcinoma
(OSCC) is the most frequent presenting high rates of morbidity and mortality, and oral
verrucous carcinoma (OVC), which exhibits a behavior distinct from a low grade variant of oral
squamous cell carcinoma. The development and progression of these malignant neoplasms are
related to the imbalance in the regulation of cell division and death associated to the tumor
microenvironment. CLIC4 protein is related to the regulation of the cell cycle, being
supraregulated in response to apoptosis and also participating in the process of
transdifferentiation of fibroblasts in cancer-associated fibroblasts (CAF), which now express αSMA.
CAFs are the main cellular components of the tumor microenvironment, having been
related to the aggressiveness and prognosis of several tumors. The aim of this study was to
evaluate the immunoexpression of CLIC4 and α-SMA proteins in oral carcinomas. The sample
consisted of 20 cases of OSCC, 15 cases of OVC and 5 cases of OM. From the medical records,
clinical data regarding the age, gender and location of the lesion were collected. A
morphological analysis was performed on HE and semiquantitative immunohistochemical
expression of the CLIC4 and α-SMA proteins in samples of paraffin-shaped lesion material.
Pearson's Chi-square test and Fisher's exact test were used to verify associations. The Spearman
test was used to search for correlation. Significance was set at of 5%. Results: Among the
OSCC, the majority occurred in male patients (n = 11; 55.0%), with a mean age of
66.95 years and the most affected anatomic location was tongue (n = 9; 45.0%). The OVC was
the female sex (n = 9; 60.0%), the mean age was 61.71 years, and the alveolar ridge was the
most affected (n = 4; 26.7%) and lip mucosa (n = 4; 26.7%). For the analysis of CLIC4
expression, its cellular location was considered. Comparing the lesions for CLIC4 labeling in
the nucleus, cytoplasm and nucleus /cytoplasm of tumor epithelial cells, no significant
difference was observed. When comparing CLIC4 expression in the OSCC and OVC stroma, a
significant difference was observed (p <0.0001). The analysis of α-SMA immunostaining in
mesenchymal cells of OSCC and OVC revealed an increase in the expression of this protein in
the tumor stroma of both tumors, and no significant difference was observed between the
lesions. When comparing the immunoexpression of αSMA and CLIC4 in the stroma of OSCC
and OVC, a positive correlation was observed but not significant in OSCC (r: 0.350 and p:
0.130). In the OVC, a positive and significant correlation was observed between the CLIC4 and
α-SMA proteins (r: 0.612 and p: 0.015). In conclusion, the results of the present study suggest
that the decrease or absence of CLIC4 protein nuclear marking associated with increased
expression of this protein in the tumor stroma seems to contribute to the process of
carcinogenesis and progression of CCEO and CVO and may have an influence on expression
of α-SMA.