Avaliação da biodistribuição do pertecnetato de sódio em ratos Wistar infectados com Trypanosoma cruzi ou tratados com benzonidazol.

Abstract

The aim of this study was to evaluate the biodistribution of sodium pertechnetate (99mTcO4), used in scintigraphic examinations, in Wistar rats experimentally infected with the Y strain of Trypanosoma cruzi, the parasite causing Chagas' disease and in rats treated for 30 days with the anti-T. cruzi benznidazole. The percentage of radioactivity per gram (%ATI/g) of various organs such as brain, heart, esophagus, stomach, small intestine, colon, spleen, liver, muscle and blood was measured in an automatic gamma counter. Comparing the controls with the treated group, it was observed that the biodistribution of 99mTcO4 did not change in the organs of animals treated with benznidazole. The rats infected with T. cruzi had increasing uptake of 99mTcO4 in the blood and decreasing in the colon. Additionally, histopathological changes in the colon were observed. In conclusion, the data show that treatment with benznidazole in rats does not change the biodistribution of 99mTcO4 but the infection by T. cruzi does it. This findings may result in potential diagnostic and clinical implications. The completion of this study had multidisciplinary nature with the involvement of biologists, physicians, pharmacists and statisticians.