masterThesis
Infecção por tripanossomatídeos em quirópteros capturados no município de Natal-RN
Fecha
2021-02-25Registro en:
SILVA, Ana Clara Oliveira da. Infecção por tripanossomatídeos em quirópteros capturados no município de Natal-RN. 2021. 103f. Dissertação (Mestrado em Biologia Parasitária) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2021.
Autor
Silva, Ana Clara Oliveira da
Resumen
Trypanosomatids are parasite protozoa belonging to the Trypanosomatidae family, which
inhere Trypanosoma cruzi and Leishmania infantum, etiologic parasites of Chagas
disease and visceral leishmaniasis. Chiropterans are ancient and natural hosts of T. cruzi
and L. infantum. However, the participation of bats in the epidemiological cycle of T. cruzi
and L. infantum is still underestimated. The aim of this study was to evaluate the infection
rate by trypanosomatids in chiropterans captured in the city of Natal-RN. Ninety-five bats
were collected together with Zoonosis Control Center (CCZ) of Natal between 2019 and
2020, 50 animals from passive capture in urban areas and 45 obtained from active
capture, using fog nets, in an environmental conservation area (Parque das Dunas) in the
municipality of Natal-RN. Animals were identified, euthanized, and fragments of liver,
spleen and skin were collected. DNA was extracted and PCR for T. cruzi kDNA and hsp70
which detects the Leishmania genus were performed. The species Artibeus planirostris
was the most prevalent among bats captured in urban environments and Parque das
Dunas, presented 95% and 56% of infection by Leishmania spp. and T. cruzi, respectively.
The total rate of bats infection by T. cruzi, Leishmania spp., and co-infected T.
cruzi/Leishmania spp. was 56% (53/95), 73% (69/95) and 41% (39/95), respectively.
Leishmania spp. was detected into the skin of 55% (43/79) of animals evaluated. Animals
captured in Parque das Dunas showed a higher infection rate by T. cruzi (67%)
(p=0,0401), when compared to bats captured in the urban environment (46%). Sex and
eating habits of chiropterans did not influence the infection rate by T. cruzi. Fruit-eating
chiropterans (90%) had a higher rate of infection by Leishmania spp., when compared to
insectivores (65%) (p=0,0067) and nectarivores (33%) (p=0,0005). On the other hand,
sex (p> 0.9999) and place of capture (urban vs. Parque das Dunas) (p = 0.3587) did not
influence the infection rate by Leishmania spp. Our results demonstrate a high infection
rate by trypanosomatids in chiropterans captured in the city of Natal-RN, indicating that
they could participate in the epidemiological transmission cycle of Leishmania spp. and T.
cruzi.