masterThesis
Efeito de um extrato de Artemísia Vulgaris L. marcação in vitro de constituintes sanguíneos com tecnécio-99m em ratos wistar
Efeito de um extrato de Artemísia Vulgaris L. marcação in vitro de constituintes sanguíneos com tecnécio-99m em ratos wistar
Fecha
2008-06-11Registro en:
TERRA, Danielle Amorim. Efeito de um extrato de Artemísia Vulgaris L. marcação in vitro
de constituintes sanguíneos com tecnécio-99m em ratos wistar. 2008. 52 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal do Rio Grande do Norte, Natal, 2008.
Autor
Terra, Danielle Amorim
Resumen
Artemisia vulgaris L..is used in folk medicine and in Traditional Chinese Medicine (TCM). This medicinal plant has been utilized as anticonvulsive, analgesic, antispasmodic effect, rheumatic pains, menstrual dyspepsia, asthenia, epilepsy, hepatitis,
fevers, anemia and to expel parasites. In nuclear medicine, blood constituents are labeled with technetium-99m (99mTc) and used as radiopharmaceuticals (radiobiocomplexes).
Authors have been described that synthetic and/or natural drugs could modify the labeling of blood constituents with 99mTc. The aim of this work was to evaluate the effects of an
aqueous extract of Artemisia vulgaris L. on the labeling of blood constituents with 99mTc. Blood samples withdrawn of Wistar rats were incubated with Artemisia vulgaris L, stannous
chloride and 99mTc, as pertechnetate ion. Aliquots of plasma (P) and blood cells (BC) were isolated. Aliquots of P and BC were also precipitated with trichloroacetic acid and soluble
(SF) and insoluble (IF) fractions were separated. The radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) were calculated. Artemisia vulgaris L.
extract decreased significantly (p<0.05) the %ATI on BC and on IF-BC. The analysis of the
results indicates that the extract could have substances that could interfere on the transport
of stannous through the erythrocyte membrane altering the labeling of blood cells with
99mTc. Working in this study was a multidisciplinary group, with Phisical therapists,
Biomedicals, Physicals, Pharmacists, Biologists, Statistics and Physicians.