doctoralThesis
Caracterização interação molecular da proteína reprimida por auxina em resposta ao controle de desenvolvimento de tomateiro
Fecha
2020-11-20Registro en:
GOMES, Géssica Laize Berto. Caracterização interação molecular da proteína reprimida por auxina em resposta ao controle de desenvolvimento de tomateiro. 2020. 104f. Tese (Doutorado em Bioquímica) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2020.
Autor
Gomes, Géssica Laize Berto
Resumen
Flowering is an important process for the plants and it corresponds to the apical
meristem transition from the vegetative to the reproductive phase. Previous data
from our laboratory using subtractive libraries identified a sequence that had
homology to AUXIN REPRESS PROTEIN (ARP). This protein has been identified
in other species but it is now clear which its function in plants is. Then, T the aim
of this work was to understand the role of ARP protein in tomato and in flowering
transition. First, it was done a sequence alignment using 13 protein sequences
and omega clustal. In this alignment it was observed that the tomato ARP protein
had the four domains that were characteristic from this family. The domain I is a
leucine-rich motif, which has an amphiphilic repression region at its N-terminus.
Domain II that contains an internal motif core composed of glycine, tryptophan
and proline. Furthermore, at the C-terminal region, there are the domain III and
IV, which together form the PB1 domain (Phox / Bem1p). This domain has a
typical lysine and a series of acid residues. Using the STRING 10.0 database
(http://www.string-dg.org) with a confidence index of 0.7, it was observed an
interaction network where ARP1 protein interacts with more than 21 different
proteins. The proteins CBS, Myf5, APE1 and GRX1 proteins, directly interact to
ARP1 protein in this network. Moreover, these proteins are also expressed in
tomato reproductive tissues as it was observed using the eFPBar tool
(http://bar.utoronto.ca/efp_tomato/cgi -bin/efpWeb.cgi). In addition, using two
hybrids tool, it was observed eight different protein-protein interactions in vivo.
Three of these sequences codify two putative proteases and the third codify the
CYTOCHROME C-OXIDASE protein. These proteins may be associated to cell
energy metabolism. The other five sequences identified codify the PHYTOEN
SYNTHASE protein. In order to better understand the possible role of ARP1
protein, then, it was used the Cytoscape 3.7.2 program in order to make an
interactome network using the in silico and in vivo data. It was possible to identify
the presence of five clusters, which are associated to plant energy metabolism,
growth mechanism and cell differentiation. Moreover, it was evaluated the auxin
levels in transgenic plants having the overexpression cassette in sense and
antisense orientation. In addition, it was observed that transgenic plants having
the antisense cassette had lower auxin levels. Considering this data and the
interactome, it may proposed a role in plant development and flowering transition
for the ARP1 protein.