XPA deficiency affects the ubiquitin-proteasome system function

dc.creatorLeal, Angélica Maria de Sousa
dc.creatorMedeiros, Lázaro Batista de Azevedo
dc.creatorMuñoz-Cadavid, Cesar Orlando
dc.creatorOliveira, Riva de Paula
dc.creatorTimóteo, Ana Rafaela de Souza
dc.creatorOliveira, Ana Helena Sales de
dc.creatorFaustino, André Luis Fonseca
dc.creatorSilva, Vandeclécio Lira da
dc.creatorSouza, Sandro José de
dc.creatorLajus, Tirzah Braz Petta
dc.creatorCampos, Julliane Tamara Araújo de Melo
dc.creatorAgnez-Lima, Lucymara Fassarella
dc.date2020-07-23T17:36:23Z
dc.date2020-07-23T17:36:23Z
dc.date2020-07
dc.identifierLEAL, Angélica Maria de Sousa; MEDEIROS, Lázaro Batista de Azevedo; MUÑOZ-CADAVID, Cesar Orlando; OLIVEIRA, Riva de Paula; TIMÓTEO, Ana Rafaela de Souza; OLIVEIRA, Ana Helena Sales de; FAUSTINO, André Luis Fonseca; SILVA, Vandeclécio Lira da; SOUZA, Sandro José de; LAJUS, Tirzah Braz Petta. XPA deficiency affects the ubiquitin-proteasome system function. Dna Repair, [S.L.], v. 94, p. 102937, out. 2020. http://dx.doi.org/10.1016/j.dnarep.2020.102937. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1568786420301865?via%3Dihub. Acesso em: 23 jul. 2020.
dc.identifierhttps://repositorio.ufrn.br/jspui/handle/123456789/29716
dc.identifier10.1016/j.dnarep.2020.102937
dc.descriptionXeroderma pigmentosum complementation group A (XPA), is defective in xeroderma pigmentosum patients, causing pre-disposition to skin cancer and neurological abnormalities, which is not well understood. Here, we analyzed the XPA-deficient cells transcriptional profile under oxidative stress. The imbalance in of ubiquitin-proteasome system (UPS) gene expression was observed in XPA-deficient cells and the involvement of nuclear factor erythroid 2-related factor-2 (NFE2L2) was indicated. Co-immunoprecipitation assays showed the interaction between XPA, apurinic-apyrimidinic endonuclease 1 (APE1) and NFE2L2 proteins. Decreased NFE2L2 protein expression and proteasome activity was also observed in XPA-deficient cells. The data suggest the involvement of the growth arrest and DNA-damage-inducible beta (GADD45β) in NFE2L2 functions. Similar results were obtained in xpa-1 (RNAi) Caenorhabditis elegans suggesting the conservation of XPA and NFE2L2 interactions. In conclusion, stress response activation occurs in XPA-deficient cells under oxidative stress; however, these cells fail to activate the UPS cytoprotective response, which may contribute to XPA patient’s phenotypes.
dc.languageen
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Brazil
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/br/
dc.rightsLOCKSS system has permission to collect, preserve, and serve this Archival Unit
dc.subjectXeroderma pigmentosum
dc.subjectOxidative stress
dc.subjectXeroderma Pigmentosum Group A Protein
dc.subjectApurinic-apyrimidinic endonuclease 1 (APE1)
dc.subjectNF-E2-Related Factor 2
dc.subjectTranscriptional regulation
dc.subjectProteolysis
dc.subjectProteostasis
dc.subjectProteasome
dc.titleXPA deficiency affects the ubiquitin-proteasome system function
dc.typearticle


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