masterThesis
Estudo da fauna culicidiana e arbovírus circulantes na unidade de conservação Flona Açu-RN
Fecha
2016-01-28Registro en:
FREIRE, Renato César de Melo. Estudo da fauna culicidiana e arbovírus circulantes na unidade de conservação Flona Açu-RN. 2016. 54f. Dissertação (Mestrado em Biologia Parasitária) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2016.
Autor
Freire, Renato César de Melo
Resumen
Introduction: The Culicidae mosquitoes are fundamental in the transmission of
many pathogens giving this Diptera family great medical importance. One branch to
understanding how these diseases can be controlled is the study of the vectors that
transmit them. Therefore the importance of conducting studies that investigate these
vectors in areas not yet explored, such as protected areas, which can nestle these
pathogens. The aim of this study was to survey the species of Culicidae in the
Caatinga phytogeographic domain and the possible presence of circulating
arboviruses. Methodology: Mosquito samples were collected from September 2011
to August 2013 the Açu National Forest. Three types of collection traps were used,
ovitrap, larvitrap and Shannon trap, in order to collect the forms of eggs, larvae and
adults respectively. The samples collected were individually identified, using the
specific morphological keys. The adults underwent viral identification by the viral
RNA extraction, RT-PCR and sequencing of the virus. Results and Discussion: The
sampling collected eight genera of the Culicidae family: Aedes, Aedeomyia,
Anopheles, Coquillettidia, Culex, Haemagogus, Mansonia and Ochlerotatus. The
traps ovitrap and larvitrap captured A. aegypti, A. albopictus, Haemagogus
spegazzini, Culex (Culex) sp, being more effective in points approaching the urban
area surrounding the Flona, of all species collected the most abundant was A.
aegypti. In Shannon trap were identified seventeen species and the most abundant
was the Ochlerotatus scapularis involved in the transmission of arbovirus Melon
virus, Ilheus virus, Rocio virus and Eastern Venezuelan Equine Encephalitis virus.
two new records belonging to subfamily Culicinae were found: Mansonia
pseudotitillans and Culex (Culex) chidesteri. The latter was found for the first time
with the Culex flavivirus virus (CxFv). The CxFv has no medical importance, it is a specific virus of the genus Culex. This data shows how much there is still to be revealed about the Caatinga area of Rio Grande do Norte. Conclusion: This study provides an alert to the preservation of protected areas, since the degradation of
these environments may result in pathogen exposure to populations, given that there
are different vectors maintained in wildlife.