Enhancing purification of chitosanase from Metarhizium anisopliae by expanded bed adsorption chromatography using Doehlert design

dc.creatorSantos, Everaldo Silvino dos
dc.creatorSantana, Sergio Carvalho de
dc.creatorFilho, Raimundo Cosme da Silva
dc.creatorOliveira, Jackson Araújo de
dc.creatorMacedo, Gorete Ribeiro de
dc.creatorPadilha, Francine Ferreira
dc.date2021-05-13T19:07:40Z
dc.date2021-05-13T19:07:40Z
dc.date2015-10-04
dc.identifierSANTANA, Sergio Carvalho de; SILVA FILHO, Raimundo Cosme da; OLIVEIRA, Jackson Araujo de; MACEDO, Gorete Ribeiro de; PADILHA, Francine Ferreira; SANTOS, Everaldo Silvino dos. Enhancing purification of chitosanase from Metarhizium anisopliae by expanded bed adsorption chromatography using Doehlert design. Biocatalysis And Agricultural Biotechnology, [S.L.], v. 4, n. 4, p. 727-736, out. 2015. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818115300050?via%3Dihub Acesso em: 06 abr. 2021. https://doi.org/10.1016/j.bcab.2015.10.005
dc.identifier1878-8181
dc.identifierhttps://repositorio.ufrn.br/handle/123456789/32524
dc.identifier10.1016/j.bcab.2015.10.005
dc.descriptionThe use of expanded bed adsorption with three different adsorbents Streamline (SP XL, DEAE and PHENYL) to purify the chitosanase from Metarhizium anisopliae strain with and without clarification using Doehlert design for the optimization of the experiments has been investigated. Chitosanases are enzymes that hydrolyze chitosan, a carbohydrate, resulting oligosaccharides that have many biological remarkable activities, such as anti-cancer, anti-HIV and anti-oxidants activities. The superficial velocity was evaluated in five levels, from 40 to 260 cm h1, while the type of adsorbent, a qualitative variable, was assessed at three levels, saving materials and time according to the Doehlert design. The results showed that it is possible to purify chitosanase from M. anisopliae by expanded bed adsorption. Chit-osanase purification factor ranged from 2 to 3.1-fold and yield ranged from 27.7 to 45.3%. The highest enzyme activity in the elution was 0.06 U/mL values which were higher than or close to those reported. The molecular mass of chitosanase was about 45 kDa using SDS-PAGE
dc.languageen
dc.publisherElsevier
dc.subjectPurification
dc.subjectExpanded bed adsorption
dc.subjectChitosanase
dc.subjectMetarhizium anisopliae
dc.subjectChitooligosaccharides
dc.titleEnhancing purification of chitosanase from Metarhizium anisopliae by expanded bed adsorption chromatography using Doehlert design
dc.typearticle


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