masterThesis
Avaliação dos efeitos do extrato de Passiflora cincinnata Masters em camundongos: efeito na ansiedade e potencial neuroprotetor
Fecha
2015-05-22Registro en:
BRANDÃO, Luiz Eduardo Mateus. Avaliação dos efeitos do extrato de Passiflora cincinnata Masters em camundongos: efeito na ansiedade e potencial neuroprotetor. 2015. 70f. Dissertação (Mestrado em Psicobiologia) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2015.
Autor
Brandão, Luiz Eduardo Mateus
Resumen
Anxiety disorders and Parkinson’s disease (PD) affect a large portion of the
world population. Indeed, therapeutic alternatives available do not contribute to
improve most clinical conditions and/or are linked with undesirable side effects. Thus,
there is a great demand for the development of new drugs to treatment of these
diseases. Passiflora cincinnata Mast. is a native species present in several Brazilian
states, popularly known as “maracujá do mato”, “maracujá tubarão” or “maracujá
mochila”. Additionally, species of Passiflora genus are traditionally known for their
exotic flowers, edible fruits with pronounced flavor and for their sedative, tranquilizer
and anxiolytic properties reported by folk medicine. These plants possess important
organic compounds such as phenols, cyanogenic glycosides, flavonoids and alkaloids,
which are responsible for the anxiolytic, antioxidant, anti-inflammatory, antihyperglycemic,
among others activities when tested in mammals. Despite this fact,
only a few studies have been conducted to investigate the possible in vivo biological
effects of Passiflora cincinnata Mast extracts. Thereby, in this study we evaluated the
effects of the alcoholic extract of this plant in anxiety and PD animal model. Mice
acutely or chronically administered with ethanolic extract of P. cincinnata do not
showed any anxiogenic- or anxyolitic-like effect in elevated plus maze (EPM). In order
to reproduce PD symptom’s in mice, we administered repeated injections of reserpine
which progressively induced motor impairments such as increase in catalepsy, oral
movements, and reduction of the average speed of the animals in the open field, as
well as depleted dopamine prodution in SNpc cells. Furthermore, this treatment
resulted in the loss of aversive memory recall in mice when undergoing PMDAT. Yet,
passiflora group also show this amnesic profile. However, animals treated
concomitantly with the alcoholic extract of Passiflora cincinnata Mast. showed higher
latency for the onset of motor impairment evaluated by catalepsy. Thus, our results
shows that the alcoholic extract of the plant P. cincinnata was able to delay the onset
of the catalepsy induced by reserpine administration, plus reverted the depletion of
dopamine production in SNpc cells.