article
Prevention of bacterial translocation using β-(1-3)-D-glucan in small bowel ischemia and reperfusion in rats
Fecha
2006Registro en:
1678-2674
Autor
Araújo Filho, Irami
Rêgo, Amália Cínthia Meneses
Pinheiro, Laíza Araújo Mohana
Azevedo, Italo Medeiros
Medeiros, Vítor Brasil
Brandão Neto, José
Medeiros, Aldo Cunha
Resumen
To investigate the role of β-(1-3)-D-glucan on 99mTc labelled Escherichia coli translocation and cytokines
secretion in rats submitted to small bowel ischemia/reperfusion injury. Methods: Five groups (n=10 each) of Wistar rats
were subjected to control(C), sham(S), group IR subjected to 45 min of bowel ischemia/60 min of reperfusion(I/R), and
group I/R+glucan subjected to 45 min of bowel ischemia/60 min of reperfusion(I/R) and injected with 2mg/Kg intramuscular.
Translocation of labelled bacteria to mesenteric lymph nodes, liver, spleen, lung and serum was determined using
radioactivity/count and colony forming units/g(CFU/g). Serum TNFα, IL-1β, IL-6, IL-10 were measured by ELISA. Results:
CFU/g and radioactivity/count were higher in I/R than in I/R+glucan rats. In C, S and S+glucan groups, bacteria and
radioactivity/count were rarely detected. The I/R+glucan rats had enhancement of IL-10 and suppressed production of
serum TNFα, IL-1β and, IL-6, compared to I/R untreated animals. Conclusion: The β-(1-3)-D-glucan modulated the
production of pro-inflammatory and anti-inflammatory cytokines during bowel ischemia/reperfusion, and attenuated
translocation of labelled bacteria