Efeito protetor do extrato de Arrabidaea chica contra o câncer de mama quimicamente induzido em modelo animal

Abstract

Objective: the aim of this study was to examine the effects of Arrabidaea chica (crajiru) extract, native plant of the Amazon, on a 7,12-dimethyl-benzanthracene (DMBA)-induced breast carcinoma model in Wistar rats. Methods: We compared the response of mammary carcinoma to the oral injected A. chica extract (ACE) for 16 weeks, associated or not with vincristine. Experimental design: normal control; DMBA group without treatment; DMBA+ACE (300 mg/kg) treatment; DMBA+vincristine. 500µg/kg injected i.p; DMBA+ACE+Vincristine 250µg/kg. Imaging using microPET and fluorescence, biochemistry, oxidative stress, hematology and histopathology were used to validate the treatments. Results: all animals survived. There was a gradual weight gain in all groups for 16 weeks, with no significant difference between them (p>0.05). The oral injection of ACE and ACE+vincristine 50% significantly reduced the incidence of breast tumors examined with PET-18FDG and fluorescence (p<0.001). Significant reduction of serum transaminases, oxidative stress and hematological toxicity were observed in these groups. Antioxidant enzyme levels in breast tissue were significantly higher compared to the DMBA and DMBA+vincristine groups. Conclusion: These results demonstrate that ACE positively influences the treatment of DMBA-induced breast cancer in animal model, suppressing abnormal cell proliferation and inducing a reduction in oxidative stress and chemotherapy toxicity. ACE may have clinical implication and be developed as a drug to reduce mortality and mitigate the toxicity of chemotherapy for breast cancer.