bachelorThesis
Avaliação da expressão proteica de nefrina em vesículas extracelulares urinárias de pacientes no pós-transplante renal
Fecha
2021-08-25Registro en:
MELLO, Clariscy Samantha Andrade da Costa. Avaliação da expressão proteica de nefrina em vesículas extracelulares urinárias de pacientes no pós-transplante renal. 2021. 55f. Trabalho de Conclusão de Curso (Graduação em Farmácia) – Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte, Natal, 2021.
Autor
Mello, Clariscy Samantha Andrade da Costa
Resumen
Introduction: Renal transplantation (KTx) is one of the main treatments for chronic
kidney disease (CKD) in terminal stage. However, some factors can cause the appearance of
albuminuria and consequent negative impact on graft survival, which can interfere with the
prognosis of post-KTx patients. The albumin/creatinine ratio (ACR) measures albuminuria, but
this marker is not specific for glomerular damage. Thus, the study aimed to evaluate the protein
expression of nephrin in urinary extracellular vesicles (uEVs) in post-KTx patients.
Methods: Forty-seven patients were recruited in their first KTx treated at the
nephrology clinic of a university hospital. Patients were followed for 3,6,9 and 12 months post KTx. Data from medical history, blood and urine samples were collected at follow-up times.
Results: The renal function parameters of the patients - ACR, creatinine and estimated
glomerular filtration rate (eGFR) - showed no significant changes (p> 0.05). Increased values
of nephrin [3 (p=0.004) and 6 months (p=0.009)] were found. Regarding the ACR assessment,
a significant increase was observed in the times of 9- (9 months x 3 months: p = 0.002; 9 months
x 6 months: 0.001) and 12 months (12 months x 3 months: p <0.001; 12 months x 6 months:
0.001) when compared to 3 and 6 months, respectively.
Conclusion: This study showed that increased nephrin expression in uEVs may
represent an important indicator of glomerular damage, being a good predictor of albuminuria
and decline in eGFR in the post- KTx.