masterThesis
Implantação da técnica de registro de células de lugar utilizando microdrives de tetrodos móveis em ratos
Fecha
2018-04-09Registro en:
PEDROSA, Rafael Hugo de Andrade. Implantação da técnica de registro de células de lugar utilizando microdrives de tetrodos móveis em ratos. 2018. 101f. Dissertação (Mestrado em Neurociências) - Universidade Federal do Rio Grande do Norte, Universidade Federal do Rio Grande do Norte, Natal, 2018.
Autor
Pedrosa, Rafael Hugo de Andrade
Resumen
The formation of spatial maps depends on the hippocampus and associated
structures. Electrophysiological activity in the CA1 region of the hippocampus
encodes spatial representations through increases in the firing rate of pyramidal
neurons, known as place cells. The present work aimed the implementation of
hippocampal electrophysiological recording technique through the use of
microdrives of multiple movable tetrodes. For this, we developed a microdrive
prototype and performed stereotaxic surgeries in rats for bilateral chronic implant.
The new prototype microdrive contained 16 movable tetrodes and allowed the
individual progressive positioning of the tetrodes in the pyramidal layer of CA1
region of the dorsal hippocampus. After the surgical recovery of the animals, we
recorded the extracellular electrophysiological activity of the CA1 region while rats
searched for water reward at the ends of a linear track. The waveforms of recorded
action potentials were then classified as individual neural units by semi-automatic
classification algorithms. Each firing of a given neuron was then associated with
the instantaneous position of the rat on the linear track, and thus we detected
place fields of the place cells. Thus, we validated the microdrive prototype
developed and, thereby, provide an important methodological basis for future
studies aiming to understand the spatial encoding of the environment and the
formation of spatial memories.