Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports

dc.creatorScherer, Robison P.
dc.creatorDallago, Rogério L.
dc.creatorPenna, Fábio G.
dc.creatorBertella, Francile
dc.creatorOliveira, Débora de
dc.creatorOliveira, Jose Vladimir de
dc.creatorPergher, Sibele Berenice Castellã
dc.date2020-06-09T01:40:50Z
dc.date2020-06-09T01:40:50Z
dc.date2012-10
dc.identifierSCHERER, Robison P.; DALLAGO, Rogério L.; PENNA, Fábio G.; BERTELLA, Francile; OLIVEIRA, Débora de; OLIVEIRA, Jose Vladimir de; PERGHER, Sibele Berenice Castellã. Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 290-294, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.003. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001004. Acesso em: 08 jun. 2020.
dc.identifier1878-8181
dc.identifierhttps://repositorio.ufrn.br/jspui/handle/123456789/29186
dc.identifier10.1016/j.bcab.2012.06.003
dc.descriptionThe use of lipases as biocatalysts has attracted much attention recently, but industrial application has been hindered mainly due to the high production cost, determined by the production yield and enzyme stability. Immobilization of biocatalysts in inert supports can ensure their use for several batches, making possible to build cost-effective processes. The objective of this work was to investigate the influence of immobilization time and enzyme to support mass ratio on the yield of immobilization and esterification activity of porcine pancreatic lipase. Higher immobilization yields (38.2%) were obtained for pillared montmorillonite, 120 min of immobilization and enzyme to support mass ratio of 2:0.5. The highest esterification activity (1403 U/g) was achieved using the montmorillonite, after 180 min of immobilization and enzyme to support ratio of 2:1. The characterization of the supports, free and immobilized enzyme on different supports made possible to elucidate the immobilization results through the knowledge of support features.
dc.languageen
dc.publisherElsevier
dc.subjectPorcine pancreatic lipase
dc.subjectImmobilization
dc.subjectKSF
dc.subjectNatural montmorillonite
dc.subjectPillared montmorillonite
dc.titleInfluence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports
dc.typearticle


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