masterThesis
Avaliação da suplementação de vitamina E sobre a concentração de alfa-tocoferol no leite materno em mulheres com partos prematuros
Fecha
2015-05-05Registro en:
PIRES, Jeane Franco. Avaliação da suplementação de vitamina E sobre a concentração de alfa-tocoferol no leite materno em mulheres com partos prematuros. 2015. 76f. Dissertação (Mestrado em Bioquímica) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2015.
Autor
Pires, Jeane Franco
Resumen
The term vitamin E refers to a group of eight molecular compounds which
differ in structure and bioavailability, and the RRR-alpha-tocopherol more biologically
active form. The composition of vitamin E in breast milk undergoes variations during
lactation, colostrum and milk richer in this micronutrient compared to transitional and
mature milk. Newborns, especially premature infants are more susceptible to vitamin
E deficiency and to prevent the damage caused by this deficiency has been
proposed supplementation of neonates with this micronutrient, however, there is no
consensus to carry out this intervention. Thus, maternal supplementation with RRRalpha-tocopherol
in the postpartum period can be a good alternative to try to raise the
alpha-tocopherol levels in breast milk and therefore provide the premature newborn
adequate amounts of vitamin E. This study to evaluate the effect of supplementation
with 400 UI acetate RRR-alpha-tocopherol in women with premature births, on the
concentration of alpha-tocopherol in breast milk colostrum, transitional and mature.
The study included 89 healthy adult women were enrolled in the control group (n =
51) and supplemented group (n = 38). Blood samples were collected and milk
colostrum soon after birth (0h milk) twenty-four hours, new rate of colostrum milk was
collected (24h milk). The transitional and mature milk were collected in seven days
(7d milk) and thirty days (30d milk) after delivery, respectively. Supplementation in
the supplemented group was held after the collection of blood and 0h milk. The
alpha-tocopherol analyzes were performed by high-performance liquid
chromatography. Serum levels of alpha-tocopherol less than 516 μg/dL were
considered indicative of nutritional deficiency. The average concentration of alphatocopherol
in the serum of the control group mothers was 1159.8 ± 292.4 μg/dL and
the supplemented group was 1128.3 ± 407.2 μg/dL (p = 0.281). All women had
nutritional status in vitamin E suitable. In both groups, it was observed that the
concentration of vitamin E in colostrum milk was higher compared to transitional and
mature milk. In the supplemented group, the concentration of alpha-tocopherol in the
milk increased 60 % after supplementation, from 1339.3 ± 414.2 μg/dL (0h milk) to
2234.7 ± 997.3 μg/dL (24h milk). While the control group values in colostrum 0h and
colostrum 24h were similar (p = 0.681). In the control group the follow-on milk alphatocopherol
value was 875.3 ± 292.4 μg/dL and in the group supplemented 1352.8 ±
542.3 μg/dL, an increase of 35% in the supplemented group compared to control (p
<0.001). In mature milk alpha-tocopherol concentrations between the control group
(426.6 ± 187.5 μg/dL) and supplemented (416.4 ± 214.2 μg/dL) were similar (p =
0.853). Only 24h milk supplemented group answered the nutritional requirement of
alpha-tocopherol (4 mg/day) of the newborn. These results show that the transport of
this micronutrient for milk occurs in a controlled and limited way. Thus, the native
vitamin E supplementation increases the concentration of alpha-tocopherol in
colostrum and milk and transition does not influence the concentration in mature milk.
Only the increase in colostrum milk was sufficient to meet the nutritional requirement
of premature newborns.