dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-27T11:29:47Z | |
dc.date.accessioned | 2022-10-05T18:52:58Z | |
dc.date.available | 2014-05-27T11:29:47Z | |
dc.date.available | 2022-10-05T18:52:58Z | |
dc.date.created | 2014-05-27T11:29:47Z | |
dc.date.issued | 2013-06-27 | |
dc.identifier | PLoS ONE, v. 8, n. 6, 2013. | |
dc.identifier | 1932-6203 | |
dc.identifier | http://hdl.handle.net/11449/75719 | |
dc.identifier | 10.1371/journal.pone.0066532 | |
dc.identifier | WOS:000321150000005 | |
dc.identifier | 2-s2.0-84879540815 | |
dc.identifier | 2-s2.0-84879540815.pdf | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3924646 | |
dc.description.abstract | Supernumerary chromosomes (B chromosomes) occur in approximately 15% of eukaryote species. Although these chromosomes have been extensively studied, knowledge concerning their specific molecular composition is lacking in most cases. The accumulation of repetitive DNAs is one remarkable characteristic of B chromosomes, and the occurrence of distinct types of multigene families, satellite DNAs and some transposable elements have been reported. Here, we describe the organization of repetitive DNAs in the A complement and B chromosome system in the grasshopper species Abracris flavolineata using classical cytogenetic techniques and FISH analysis using probes for five multigene families, telomeric repeats and repetitive C0t-1 DNA fractions. The 18S rRNA and H3 histone multigene families are highly variable and well distributed in A. flavolineata chromosomes, which contrasts with the conservation of U snRNA genes and less variable distribution of 5S rDNA sequences. The H3 histone gene was an extensively distributed with clusters occurring in all chromosomes. Repetitive DNAs were concentrated in C-positive regions, including the pericentromeric region and small chromosomal arms, with some occurrence in C-negative regions, but abundance was low in the B chromosome. Finally, the first demonstration of the U2 snRNA gene in B chromosomes in A. flavolineata may shed light on its possible origin. These results provide new information regarding chromosomal variability for repetitive DNAs in grasshoppers and the specific molecular composition of B chromosomes. © 2013 Bueno et al. | |
dc.language | eng | |
dc.relation | PLOS ONE | |
dc.relation | 2.766 | |
dc.relation | 1,164 | |
dc.rights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | DNA fragment | |
dc.subject | histone H3 | |
dc.subject | repetitive DNA | |
dc.subject | RNA 18S | |
dc.subject | RNA 5S | |
dc.subject | satellite DNA | |
dc.subject | Abracris flavolineata | |
dc.subject | animal cell | |
dc.subject | animal tissue | |
dc.subject | autosome | |
dc.subject | bioaccumulation | |
dc.subject | Caelifera | |
dc.subject | centromere | |
dc.subject | chromosome analysis | |
dc.subject | chromosome map | |
dc.subject | chromosome variant | |
dc.subject | DNA sequence | |
dc.subject | female | |
dc.subject | fluorescence in situ hybridization | |
dc.subject | genetic conservation | |
dc.subject | karyotype | |
dc.subject | male | |
dc.subject | nonhuman | |
dc.subject | nucleotide sequence | |
dc.subject | plesiomorphy | |
dc.subject | RNA gene | |
dc.subject | telomere | |
dc.title | Chromosomal Mapping of Repetitive DNAs in the Grasshopper Abracris flavolineata Reveal Possible Ancestry of the B Chromosome and H3 Histone Spreading | |
dc.type | Artigo | |