dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:29:33Z
dc.date.accessioned2022-10-05T18:50:58Z
dc.date.available2014-05-27T11:29:33Z
dc.date.available2022-10-05T18:50:58Z
dc.date.created2014-05-27T11:29:33Z
dc.date.issued2013-06-01
dc.identifierMicroscopy Research and Technique, v. 76, n. 6, p. 618-624, 2013.
dc.identifier1059-910X
dc.identifier1097-0029
dc.identifierhttp://hdl.handle.net/11449/75471
dc.identifier10.1002/jemt.22208
dc.identifierWOS:000319298200008
dc.identifier2-s2.0-84878189553
dc.identifier8456490300814833
dc.identifier0000-0002-2420-2550
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3924406
dc.description.abstractThe aim of this study was to isolate, culture, and characterize mesenchymal stem cells (MSCs) from horse bone marrow (BM) using the techniques of flow cytometry, immunocytochemistry, cytogenetics, and electron microscopy. Immunophenotypic analysis revealed the presence of MSCs with high expression of the CD90 marker, lower expression of the CD44 marker, and absent expression of the CD34 marker. In assays of differentiation, the positive response to osteogenic (OST), chondrogenic (CDG), and adipogenic (ADP) differentiation signals was observed and characterized by deposition of calcium-rich extracellular matrix (OST), proteoglycans and collagen II (CDG) and intracellular deposition of fat drops (ADP). In immunocytochemical characterization, MSCs were immunopositive for CD44, vimentin, and PCNA, and they were negative for CD13. In the ultrastructural analysis of MSCs, the most outstanding characteristic was the presence of rough endoplasmic reticulum with very dilated cisterns filled with a low electrodensity material. Additionally, MSCs had normal karyotypes (2n=64) as evidenced by cytogenetic analysis, and aneuploidy in metaphase was not observed. The protocols for isolating, culturing, and characterizing equine MSCs used in this study were shown to be appropriate for the production of a cell population with a good potential for differentiation and without aneuploidy that can be used to study future cellular therapies. © 2013 Wiley Periodicals, Inc.
dc.languageeng
dc.relationMicroscopy Research and Technique
dc.relation1.087
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectDifferentiation
dc.subjectHorse
dc.subjectIsolation
dc.subjectMesenchymal stem cells
dc.subjectSurface marker
dc.subjectEquidae
dc.titleImmunophenotypic, immunocytochemistry, ultrastructural, and cytogenetic characterization of mesenchymal stem cells from equine bone marrow
dc.typeArtigo


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