dc.contributorCentro de Estudos e Pesquisa em Reprodução
dc.contributorUniversidade Estadual de Campinas (UNICAMP)
dc.contributorUniversidade Federal de Alfenas (UNIFAL)
dc.contributorCentro de Fertilização
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:25:51Z
dc.date.accessioned2022-10-05T18:26:37Z
dc.date.available2014-05-27T11:25:51Z
dc.date.available2022-10-05T18:26:37Z
dc.date.created2014-05-27T11:25:51Z
dc.date.issued2011-05-01
dc.identifierJornal Brasileiro de Reproducao Assistida, v. 15, n. 3, p. 18-21, 2011.
dc.identifier1517-5693
dc.identifier1518-0557
dc.identifierhttp://hdl.handle.net/11449/72400
dc.identifier10.1016/j.fertnstert.2011.07.942
dc.identifier2-s2.0-79958060486
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3921466
dc.description.abstractObjective: This case-control study analyzed mass spectrometry fingerprinting patterns of culture media samples used for embryo culture to predict embryo implantation. Methods: The culture medium harvested after embryo transfer of 22 embryos from 13 patients was used for the experiments. After embryo transfer, the remaining culture media were collected and samples were split in positive (n=8) and negative (n=14) implantation groups according to implantation outcomes (100% or 0% of implantation). Samples were individually diluted and injected directly to the Electrospray ionization (ESI) MS coupled to a Quadrupole Time-of-flight MS (Q-ToF-MS).Ions relative intensities of each spectrum were considered. Data analysis was conducted in MatLab 7.0 version using Partial Least Squares - Discriminant Analysis toolbox. Results: There were 3027 observed ions at 100% and 0% implantation groups by ESI-Q-ToF-MS. The statistical model could categorize the samples in two clusters, based on their positive and negative implantation outcomes. Less intense ions present in the mass spectra with statistical significance have contributed to the major differences to group distinction. Conclusions: Positive and negative implantation embryos showed a specific biochemical pattern present in culture media, which could be detected as a fast, simple and non-invasive way. This biochemical profile could help the selection of the most viable embryo, improving single embryo transfer and thus eliminating the risk and undesirable outcomes of multiple pregnancies. © Todos os direitos reservados a SBRA - Sociedade Brasileira de Reprodução Assistida.
dc.languageeng
dc.languagepor
dc.relationJornal Brasileiro de Reproducao Assistida
dc.relation0,282
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectEmbryo implantation
dc.subjectEmbryo selection
dc.subjectEmbryology
dc.subjectanalytical parameters
dc.subjectbiochemistry
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectDNA fingerprinting
dc.subjectelectrospray mass spectrometry
dc.subjectembryo
dc.subjectembryo culture
dc.subjectembryo implantation prediction
dc.subjectembryo transfer
dc.subjecthuman
dc.subjectnidation
dc.subjectoutcome assessment
dc.subjectquadrupole mass spectrometry
dc.subjectrisk assessment
dc.subjecttime of flight mass spectrometry
dc.titleValor preditivo do potencial de implantação embrionário pela análise do perfil químico de meios de cultivo por espectrometria de massas (ESI-Q-ToF)
dc.typeArtigo


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