dc.contributorFujian Medical University Affiliated Union Hospital
dc.contributorHuazhong Science and Technology University
dc.contributorCBS-KNAW Fungal Biodiversity Centre
dc.contributorUniversity of Amsterdam
dc.contributorPeking University Health Science Center
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorGIRO Technological Centre
dc.contributorIRTA
dc.date.accessioned2014-05-27T11:24:39Z
dc.date.accessioned2022-10-05T18:20:42Z
dc.date.available2014-05-27T11:24:39Z
dc.date.available2022-10-05T18:20:42Z
dc.date.created2014-05-27T11:24:39Z
dc.date.issued2010-03-23
dc.identifierMicrobial Ecology, v. 60, n. 1, p. 149-156, 2010.
dc.identifier0095-3628
dc.identifierhttp://hdl.handle.net/11449/71608
dc.identifier10.1007/s00248-010-9651-4
dc.identifier2-s2.0-77955417388
dc.identifier2-s2.0-77955417388.pdf
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3920772
dc.description.abstractBlack yeast members of the Herpotrichiellaceae present a complex ecological behavior: They are often isolated from rather extreme environments polluted with aromatic hydrocarbons, while they are also regularly involved in human opportunistic infections. A selective technique to promote the in vitro growth of herpotrichiellaceous fungi was applied to investigate their ecophysiology. Samples from natural ecological niches and man-made environments that might contain black yeasts were enriched on an inert solid support at low humidity and under a controlled atmosphere rich in volatile aromatic hydrocarbons. Benzene, toluene, and xylene were provided separately as the sole carbon and energy source via the gas phase. The assayed isolation protocol was highly specific toward mesophilic Exophiala species (70 strains of this genus out of 71 isolates). Those were obtained predominantly from creosote-treated railway ties (53 strains), but isolates were also found on wild berries (11 strains) and in guano-rich soil samples (six strains). Most of the isolates were obtained on toluene (43 strains), but enrichments on xylene and benzene also yielded herpotrichiellaceous fungi (17 and 10 isolates, respectively). Based upon morphological characterizations and DNA sequences of the full internal transcriber spacers (ITS) and the 8.5S rRNA genes, the majority of the obtained isolates were affiliated to the recently described species Exophiala xenobiotica (32 strains) and Exophiala bergeri (nine strains). Members of two other phylogenetic groups (24 and two strains, respectively) somewhat related to E. bergeri were also found, and a last group (three strains) corresponded to an undescribed Exophiala species. © 2010 The Author(s).
dc.languageeng
dc.relationMicrobial Ecology
dc.relation3.614
dc.relation1,272
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjectaromatic hydrocarbon
dc.subjectfungal DNA
dc.subjectribosomal spacer DNA
dc.subjectribosome RNA
dc.subjectclassification
dc.subjectculture medium
dc.subjectDNA sequence
dc.subjectgenetics
dc.subjectisolation and purification
dc.subjectmetabolism
dc.subjectmicrobiology
dc.subjectphylogeny
dc.subjectyeast
dc.subjectCulture Media
dc.subjectDNA, Fungal
dc.subjectDNA, Ribosomal Spacer
dc.subjectEnvironmental Microbiology
dc.subjectHydrocarbons, Aromatic
dc.subjectPhylogeny
dc.subjectRNA, Ribosomal
dc.subjectSequence Analysis, DNA
dc.subjectYeasts
dc.subjectChaetothyriales
dc.subjectExophiala
dc.subjectExophiala bergeri
dc.subjectFungi
dc.subjectHerpotrichiellaceae
dc.titleIsolation and identification of black yeasts by enrichment on atmospheres of monoaromatic hydrocarbons
dc.typeArtigo


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