dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:22:28Z
dc.date.accessioned2022-10-05T18:07:04Z
dc.date.available2014-05-27T11:22:28Z
dc.date.available2022-10-05T18:07:04Z
dc.date.created2014-05-27T11:22:28Z
dc.date.issued2007-05-18
dc.identifierGenetics and Molecular Research, v. 6, n. 1, p. 152-165, 2007.
dc.identifier1676-5680
dc.identifierhttp://hdl.handle.net/11449/69668
dc.identifier2-s2.0-34248363273
dc.identifier2-s2.0-34248363273.pdf
dc.identifier5333250355049814
dc.identifier1525665408900195
dc.identifier0000-0001-7831-1149
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3919079
dc.description.abstractThe putative eukaryotic translation initiation factor 5A (eIF5A) is an essential protein for cell viability and the only cellular protein known to contain the unusual amino acid residue hypusine. eIF5A has been implicated in translation initiation, cell proliferation, nucleocytoplasmic transport, mRNA decay, and actin polarization, but the precise biological function of this protein is not clear. However, eIF5A was recently shown to be directly involved with the translational machinery. A screen for synthetic lethal mutations was carried out with one of the temperature-sensitive alleles of TIF51A (tif51A-3) to identify factors that functionally interact with eIF5A and revealed the essential gene YPT1. This gene encodes a small GTPase, a member of the rab family involved with secretion, acting in the vesicular trafficking between endoplasmatic reticulum and the Golgi. Thus, the synthetic lethality between TIF51A and YPT1 may reveal the connection between translation and the polarized distribution of membrane components, suggesting that these proteins work together in the cell to guarantee proper protein synthesis and secretion necessary for correct bud formation during G1/ S transition. Future studies will investigate the functional interaction between eIF5A and Ypt1 in order to clarify this involvement of eIF5A with vesicular trafficking. ©FUNPEC-RP.
dc.languageeng
dc.relationGenetics and Molecular Research
dc.relation0,439
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjecteIF5A
dc.subjectGenetic interaction
dc.subjectSynthetic lethality
dc.subjectVesicular trafficking
dc.subjectYpt1
dc.subjectguanosine triphosphatase
dc.subjectinitiation factor 5A
dc.subjectRab protein
dc.subjectsynthetic DNA
dc.subjectallele
dc.subjectbudding
dc.subjectcell proliferation
dc.subjectgene identification
dc.subjectgene mutation
dc.subjectgenetic screening
dc.subjectlethal gene
dc.subjectnonhuman
dc.subjectnucleocytoplasmic transport
dc.subjectprotein secretion
dc.subjectprotein synthesis
dc.subjectSaccharomyces cerevisiae
dc.subjecttranslation initiation
dc.subjectG1 Phase
dc.subjectGenes, Lethal
dc.subjectMutation
dc.subjectPeptide Initiation Factors
dc.subjectrab GTP-Binding Proteins
dc.subjectRNA-Binding Proteins
dc.subjectS Phase
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectTransport Vesicles
dc.subjectEukaryota
dc.titleUse of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae
dc.typeArtigo


Este ítem pertenece a la siguiente institución