Computer-assisted analysis of p53 and PCNA expression in oral lesions infected with human papillomavirus

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorInstituto Adolfo Lutz (IAL)
dc.date.accessioned2014-05-27T11:20:36Z
dc.date.accessioned2022-10-05T17:48:45Z
dc.date.available2014-05-27T11:20:36Z
dc.date.available2022-10-05T17:48:45Z
dc.date.created2014-05-27T11:20:36Z
dc.date.issued2003-02-01
dc.identifierAnalytical and Quantitative Cytology and Histology, v. 25, n. 1, p. 19-24, 2003.
dc.identifier0884-6812
dc.identifierhttp://hdl.handle.net/11449/67192
dc.identifier2-s2.0-0037324480
dc.identifier1768025290373669
dc.identifier0000-0003-1740-7360
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3916873
dc.description.abstractOBJECTIVE: To carry out a retrospective study to determine whether human papillomavirus (HPV) infection and immunohistochemical expression of p53 and proliferating cell nuclear antigen (PCNA) are related to the risk of oral cancer. STUDY DESIGN: Fifty-seven oral biopsies, consisting of 30 oral squamous papillomas (OSPs) and 27 oral squamous cell carcinomas (OSCCs) were tested for the presence of HPV 6/11 and 16/18 by in situ hybridization using catalyzed signal amplification and in situ hybridization. p53 And PCNA expression was analyzed by immunohistochemistry and evaluated quantitatively by image analysis. RESULTS: Nineteen of the 57 oral lesions (33.3%) were positive for HPV. HPV 6/11 was found in 6 of 30 (20%) OSPs and 1 of 27 (3.7%) OSCCs. HPV 16/18 was found in 10 of 27 (37%) OSCCs and 2 of 30 (6.7%) OSPs. Sixteen of the 19 HPV-positive cases (84.2%) were p53 negative; 5 (9%) were HPV 6/11 and 11 (19%) HPV 16/18, with an inverse correlation between the presence of HPV DNA and p53 expression (P=.017, P < .05). PCNA expression appeared in 18 (94.7%) of HPV positive cases, showing that HPV 16/18 was associated with intensity of PCNA expression and with OSCCs (P=.037, P < .05). CONCLUSION: Quantitative evaluation of p53 by image analysis showed an inverse correlation between p53 expression and HPV presence, suggesting protein degradation. Image analysis also demonstrated that PCNA expression was more intense in HPV DNA 16/18 OSCCs. These findings suggest involvement of high-risk HPV types in oral carcinogenesis.
dc.languageeng
dc.relationAnalytical and Quantitative Cytology and Histology
dc.relation0.398
dc.relation0,227
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectImage analysis, computer-assisted
dc.subjectp53 protein
dc.subjectPapillomavirus, human
dc.subjectProliferating cell
dc.subjectcycline
dc.subjectprotein p53
dc.subjectvirus DNA
dc.subjectbiopsy
dc.subjectcancer diagnosis
dc.subjectcancer risk
dc.subjectcarcinogenesis
dc.subjectcatalysis
dc.subjectclinical article
dc.subjectcomputer analysis
dc.subjectcontrolled study
dc.subjectcorrelation analysis
dc.subjectgene amplification
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectimage analysis
dc.subjectimmunohistochemistry
dc.subjectin situ hybridization
dc.subjectmouth cancer
dc.subjectpapilloma
dc.subjectpriority journal
dc.subjectprotein degradation
dc.subjectprotein expression
dc.subjectquantitative assay
dc.subjectretrospective study
dc.subjectsquamous cell carcinoma
dc.subjectvirus infection
dc.subjectWart virus
dc.subjectCarcinoma, Squamous Cell
dc.subjectImage Processing, Computer-Assisted
dc.subjectImmunoenzyme Techniques
dc.subjectIn Situ Hybridization
dc.subjectMouth Neoplasms
dc.subjectPapilloma
dc.subjectPapillomaviridae
dc.subjectPapillomavirus Infections
dc.subjectProliferating Cell Nuclear Antigen
dc.subjectRetrospective Studies
dc.subjectTumor Markers, Biological
dc.subjectTumor Suppressor Protein p53
dc.subjectTumor Virus Infections
dc.subjectDNA viruses
dc.subjectHuman papillomavirus
dc.subjectHuman papillomavirus type 16
dc.subjectHuman papillomavirus types
dc.subjectPapillomavirus
dc.titleComputer-assisted analysis of p53 and PCNA expression in oral lesions infected with human papillomavirus
dc.typeArtigo


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