Artigo
Application of on-line C30 RP-HPLC-NMR for the analysis of flavonoids from leaf extract of Maytenus aquifolium
Fecha
2000-10-05Registro en:
Phytochemical Analysis, v. 11, n. 5, p. 317-321, 2000.
0958-0344
10.1002/1099-1565(200009/10)11:5<317::AID-PCA529>3.0.CO;2-K
WOS:000089517700007
2-s2.0-0033824976
7927877224326837
0000-0003-3032-2556
Autor
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Institut für Organische Chemie
Resumen
The application of on-line C30-reversed-phase high-pressure liquid chromatography-nuclear magnetic resonance spectroscopy is described for the analysis of tetraglycosylated flavonoids in aqueous and hydroalcoholic extracts of the leaves of Maytenus aquifolium (Celastraceae). Triacontyl stationary phases showed adequate separation for on-line 1H-NMR measurements at 600 MHz and allowed the characterisation of these flavonoids by detection of both aromatic and anomeric proton signals. Copyright (C) 2000 John Wiley and Sons, Ltd.