dc.contributorUniv. Estadual de Londrina 86051-970
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorLnstitut Pasteur, 25, R. Dr. R.
dc.contributorINRA, la Minière, 78285 Cedex
dc.date.accessioned2014-05-27T11:19:53Z
dc.date.accessioned2022-10-05T17:41:16Z
dc.date.available2014-05-27T11:19:53Z
dc.date.available2022-10-05T17:41:16Z
dc.date.created2014-05-27T11:19:53Z
dc.date.issued2000-03-01
dc.identifierFEMS Microbiology Ecology, v. 31, n. 3, p. 255-259, 2000.
dc.identifier0168-6496
dc.identifierhttp://hdl.handle.net/11449/66123
dc.identifier10.1111/j.1574-6941.2000.tb00691.x
dc.identifierWOS:000086001100009
dc.identifier2-s2.0-0342470947
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3915954
dc.description.abstractThe survival and conjugation ability of sporogenic and asporogenic Bacillus thuringiensis strains were investigated in broth, in non-amended sterile clay soil monoculture and in mixed soil culture. The 75 kb pHT73 plasmid carrying an erythromycin resistance determinant and a cry1Ac gene was transferred in mating broth and soil microcosm. Survival of strains was assessed in soil monoculture and in mixed soil culture for up to 20 days. Sporogenic strains rapidly formed viable spores which were maintained until the end of the experiment. The asporogenic strains were no longer recovered after 8 days of incubation. This study shows that the environmental impact of asporogenic B. thuringiensis strains is lower than that of sporogenic B. thuringiensis strains. Thus, the use of asporogenic strains may significantly reduce any potential risk (gene transfer, soil and plant contamination) due to the dissemination of B. thuringiensis-based biopesticides in the environment. Copyright (C) 2000 Federation of European Microbiological Societies.
dc.languageeng
dc.relationFEMS Microbiology Ecology
dc.relation3.495
dc.relation1,492
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectBacillus thuringiensis
dc.subjectConjugation
dc.subjectPlasmid
dc.subjectSoil microcosm
dc.subjectSporulation mutant
dc.subjectmicrocosm
dc.subjectsoil microorganism
dc.subjectsurvival
dc.subjectbacterium conjugation
dc.subjectecosystem
dc.subjectgene transfer
dc.subjectmating
dc.subjectmutant
dc.subjectnonhuman
dc.subjectplasmid
dc.subjectpriority journal
dc.subjectsoil
dc.subjectsporogenesis
dc.titleSurvival and conjugation of Bacillus thuringiensis in a soil microcosm
dc.typeArtigo


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