dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:19:45Z
dc.date.accessioned2022-10-05T17:39:05Z
dc.date.available2014-05-27T11:19:45Z
dc.date.available2022-10-05T17:39:05Z
dc.date.created2014-05-27T11:19:45Z
dc.date.issued1999-07-01
dc.identifierFEMS Immunology and Medical Microbiology, v. 24, n. 3, p. 379-382, 1999.
dc.identifier0928-8244
dc.identifierhttp://hdl.handle.net/11449/65801
dc.identifier10.1016/S0928-8244(99)00057-7
dc.identifierWOS:000081004800020
dc.identifier2-s2.0-0032782903
dc.identifier2-s2.0-0032782903.pdf
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3915680
dc.description.abstractThe isolation of Clostridium botulinum from honey samples is described. Botulism is characterized as an intoxication provoked by ingestion of contaminated foods with this toxin. Infant botulism happens by the ingestion of spores of C. botulinum together with food that in special conditions of the intestinal tract, such as those present in babies of less than 1 year old, will allow the germination and colonization of the intestine with production and absorption of botulinic toxin. The samples were subjected to dilution and to a thermal shock and cultivated in modified CMM (Difco). Cultures were subjected to Gram smears and toxicity tests in mice. The toxic cultures were purified in RFCA (Oxoid) plates and incubated in anaerobic jars. Positive samples were typed using the mouse assay neutralization test. From the 85 honey samples analyzed, six were positive for C. botulinum (7.06%), and identified as producers of type A, B, and D toxins.
dc.languageeng
dc.relationFEMS Immunology and Medical Microbiology
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectClostridium botulinum
dc.subjectHoney
dc.subjectInfant botulism
dc.subjectToxin
dc.subjectbotulinum toxin
dc.subjectbacterial colonization
dc.subjectbacterial spore
dc.subjectbacterium culture
dc.subjectbacterium isolation
dc.subjectBrazil
dc.subjectdilution
dc.subjectfood contamination
dc.subjectgermination
dc.subjectGram staining
dc.subjecthoney
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjecttoxicity testing
dc.subjectAnimals
dc.subjectMice
dc.subjectSpores, Bacterial
dc.titleStudy of the presence of the spores of Clostridium botulinum in honey in Brazil
dc.typeArtigo


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