dc.contributorUniversidade Estadual de Campinas (UNICAMP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:18:09Z
dc.date.accessioned2022-10-05T17:32:42Z
dc.date.available2014-05-27T11:18:09Z
dc.date.available2022-10-05T17:32:42Z
dc.date.created2014-05-27T11:18:09Z
dc.date.issued1996-12-01
dc.identifierAnalytical Sciences, v. 12, n. 3, p. 443-447, 1996.
dc.identifier0910-6340
dc.identifierhttp://hdl.handle.net/11449/64933
dc.identifier10.2116/analsci.12.443
dc.identifier2-s2.0-0030510001
dc.identifier2-s2.0-0030510001.pdf
dc.identifier8540599256820672
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3914907
dc.description.abstractA new methodology for soluble oxalic acid determination in grass samples was developed using a two enzyme reactor in an FIA system. The reactor consisted of 3 U of oxalate oxidase and 100 U of peroxidase immobilized on Sorghum vulgare seeds activated with glutaraldehyde. The carbon dioxide was monitored spectrophotometrically, after reacting with an acid-base indicator (Bromocresol Purple) after it permeated through a PTFE membrane. A linear response range was observed between 0.25 and 1.00mmol l-1 of oxalic acid; the data was fit by the equation A=-0.8(±1.5)+ 57.2(±2.5)[oxalate], with a correlation coefficient of 0.9971 and a relative standard deviation of 2% for n=5. The variance for a 0.25 mmol l-1 oxalic acid standard solution was lower than 4% for 11 measurements. The FIA system allows analysis of 20 samples per hour without prior treatment. The proposed method showed a good correlation with that of the Sigma Kit.
dc.languageeng
dc.relationAnalytical Sciences
dc.relation1.355
dc.relation0,424
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjectGrass
dc.subjectOxalate determination
dc.subjectOxalate oxidase
dc.subjectSorghum vulgare seeds
dc.titleDetermination of Oxalate in Grass by FIA with a Spectrophotometric Detection System Using a Two Enzyme Reactor
dc.typeArtigo


Este ítem pertenece a la siguiente institución