dc.contributor | Universidade Estadual de Campinas (UNICAMP) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-27T11:18:09Z | |
dc.date.accessioned | 2022-10-05T17:32:42Z | |
dc.date.available | 2014-05-27T11:18:09Z | |
dc.date.available | 2022-10-05T17:32:42Z | |
dc.date.created | 2014-05-27T11:18:09Z | |
dc.date.issued | 1996-12-01 | |
dc.identifier | Analytical Sciences, v. 12, n. 3, p. 443-447, 1996. | |
dc.identifier | 0910-6340 | |
dc.identifier | http://hdl.handle.net/11449/64933 | |
dc.identifier | 10.2116/analsci.12.443 | |
dc.identifier | 2-s2.0-0030510001 | |
dc.identifier | 2-s2.0-0030510001.pdf | |
dc.identifier | 8540599256820672 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3914907 | |
dc.description.abstract | A new methodology for soluble oxalic acid determination in grass samples was developed using a two enzyme reactor in an FIA system. The reactor consisted of 3 U of oxalate oxidase and 100 U of peroxidase immobilized on Sorghum vulgare seeds activated with glutaraldehyde. The carbon dioxide was monitored spectrophotometrically, after reacting with an acid-base indicator (Bromocresol Purple) after it permeated through a PTFE membrane. A linear response range was observed between 0.25 and 1.00mmol l-1 of oxalic acid; the data was fit by the equation A=-0.8(±1.5)+ 57.2(±2.5)[oxalate], with a correlation coefficient of 0.9971 and a relative standard deviation of 2% for n=5. The variance for a 0.25 mmol l-1 oxalic acid standard solution was lower than 4% for 11 measurements. The FIA system allows analysis of 20 samples per hour without prior treatment. The proposed method showed a good correlation with that of the Sigma Kit. | |
dc.language | eng | |
dc.relation | Analytical Sciences | |
dc.relation | 1.355 | |
dc.relation | 0,424 | |
dc.rights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | Grass | |
dc.subject | Oxalate determination | |
dc.subject | Oxalate oxidase | |
dc.subject | Sorghum vulgare seeds | |
dc.title | Determination of Oxalate in Grass by FIA with a Spectrophotometric Detection System Using a Two Enzyme Reactor | |
dc.type | Artigo | |