dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorEscola Paulista de Medicina
dc.date.accessioned2014-05-27T11:18:07Z
dc.date.accessioned2022-10-05T17:32:13Z
dc.date.available2014-05-27T11:18:07Z
dc.date.available2022-10-05T17:32:13Z
dc.date.created2014-05-27T11:18:07Z
dc.date.issued1996-10-19
dc.identifierJournal of Medical and Veterinary Mycology, v. 34, n. 4, p. 265-272, 1996.
dc.identifier0268-1218
dc.identifierhttp://hdl.handle.net/11449/64864
dc.identifier10.1080/02681219680000451
dc.identifierWOS:A1996VC30200006
dc.identifier2-s2.0-0029833646
dc.identifier2-s2.0-79961136344
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3914846
dc.description.abstractThe exoantigen of Paracoccidioides brasiliensis standardized by Camargo et al. [1] (AgR) was used to evaluate the in vivo and in vitro cell immune response of experimental animals and of patients with paracoccidioidomycosis (PBM). Fava Netto antigen (AgF) was tested in parallel as a control antigen. The study was conducted with mice and guinea pigs infected with P. brasiliensis or immunized with its fungal antigens, on patients with PBM and on their respective control groups. The cell immune response was analysed by skin tests, and by the macrophage and leucocyte migration inhibition tests (MMIT and LMIT) in the animals and in the patients, respectively. The skin test with AgR as paracoccidioidin was positive in infected or immunized mice and guinea pigs and negative in control animals. The skin tests with AgR (24 h) showed 96.7% positivity in patients with PBM and were negative in control individuals. Histopathological study of the in vivo tests in the different experimental models was consistent with a delayed hypersensitivity response (DHR). Immunohistochemical study of the skin tests of PBM patients demonstrated a predominance of T lymphocytes, confirming the nature of a DHR to the fungal antigens. The in vitro cell immune response showed variable results for the various experimental models, i.e. significant rates of MMIT in immunized mice, a tendency to positivity in infected guinea pigs, and the absence of migration inhibition in PBM patients. Taken together, the data indicate that the AgR is efficient as paracoccidioidin in the evaluation of DHR in PBM, with an optimum time of reading the test of 24 h.
dc.languageeng
dc.relationJournal of Medical and Veterinary Mycology
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectParacoccidioides brasiliensis
dc.subjectparacoccidioidin
dc.subjectparacoccidioidomycosis
dc.subjectfungus antigen
dc.subjectallergenicity
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectantigen detection
dc.subjectdelayed hypersensitivity
dc.subjectguinea pig
dc.subjectimmune response
dc.subjectmacrophage migration
dc.subjectmale
dc.subjectmouse
dc.subjectnonhuman
dc.subjectparacoccidioides brasiliensis
dc.subjectskin allergy
dc.subjectskin test
dc.subjectsouth american blastomycosis
dc.subjectt lymphocyte activation
dc.subjectAnimals
dc.subjectAntigens, Fungal
dc.subjectDermatitis, Allergic Contact
dc.subjectGuinea Pigs
dc.subjectHumans
dc.subjectImmunity, Cellular
dc.subjectLymphocytes
dc.subjectMacrophages
dc.subjectMale
dc.subjectMice
dc.subjectParacoccidioides
dc.subjectParacoccidioidomycosis
dc.subjectReference Values
dc.subjectSkin
dc.subjectSkin Tests
dc.subjectAnimalia
dc.subjectCavia
dc.subjectCavia porcellus
dc.subjectFava
dc.subjectFungi
dc.subjectparacoccidioidomycosis brasiliensis
dc.subjectSus scrofa
dc.titleEvaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis
dc.typeArtigo


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