Evaluation of an indirect ELISA method for the detection of chicken antibodies against infectious bronchitis virus

dc.contributorMEC
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorSOLVAY Saúde Animal
dc.date.accessioned2014-05-27T11:18:04Z
dc.date.accessioned2022-10-05T17:31:16Z
dc.date.available2014-05-27T11:18:04Z
dc.date.available2022-10-05T17:31:16Z
dc.date.created2014-05-27T11:18:04Z
dc.date.issued1996-01-01
dc.identifierRevista de Microbiologia, v. 27, n. 1, p. 64-69, 1996.
dc.identifier0001-3714
dc.identifierhttp://hdl.handle.net/11449/64733
dc.identifier2-s2.0-0006233733
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3914733
dc.description.abstractAn indirect ELISA method was developed and applied to detect antibodies against infectious bronchitis virus (IBV). Three hundred serum samples collected from vaccinated and non-vaccinated chickens under field conditions were tested. The ELISA results were compared with antibody titers determined by a serum neutralization assay (SN). Good coefficients of correlation (r2 = 0.750) and of agreement (80%) were recorded and the ELISA reproducibility was high. The cutoff point between those techniques corresponded to ELISA level 2. It is concluded that our indirect ELISA method can advantageously replace the conventional SN test, allowing a good follow-up of IBV post-vaccination antibodies due to its convenience and applicability compared to the other assay.
dc.languageeng
dc.relationRevista de Microbiologia
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectELISA
dc.subjectInfectious Bronchitis Virus (IBV)
dc.subjectPost-vaccine serological monitoring
dc.titleEvaluation of an indirect ELISA method for the detection of chicken antibodies against infectious bronchitis virus
dc.typeArtigo


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