Artigo
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
Fecha
2012-11-01Registro en:
International Journal of Tuberculosis and Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 16, n. 11, p. 1535-1537, 2012.
1027-3719
10.5588/ijtld.11.0658
WOS:000310173900020
2114570774349859
Autor
Inst Nacl Saúde Dr Ricardo Jorge INSA
Cumura Hosp
Hosp Fernando Fonseca
Univ Evora
Universidade Estadual Paulista (Unesp)
Inst Nacl de Pesquisas da Amazonia
Univ Nova Lisboa
Resumen
The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair ppoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.