dc.contributor | Ctr Dis Control & Prevent | |
dc.contributor | Universidade Estadual de Maringá (UEM) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Instituto Adolfo Lutz (IAL) | |
dc.contributor | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2014-05-20T15:27:12Z | |
dc.date.accessioned | 2022-10-05T16:41:45Z | |
dc.date.available | 2014-05-20T15:27:12Z | |
dc.date.available | 2022-10-05T16:41:45Z | |
dc.date.created | 2014-05-20T15:27:12Z | |
dc.date.issued | 2004-09-01 | |
dc.identifier | Antimicrobial Agents and Chemotherapy. Washington: Amer Soc Microbiology, v. 48, n. 9, p. 3373-3381, 2004. | |
dc.identifier | 0066-4804 | |
dc.identifier | http://hdl.handle.net/11449/37226 | |
dc.identifier | 10.1128/AAC.48.9.3373-3381.2004 | |
dc.identifier | WOS:000223625800024 | |
dc.identifier | WOS000223625800024.pdf | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3908789 | |
dc.description.abstract | We investigated mutations in the genes katG, inhA (regulatory and structural regions), and kasA and the oxyR-ahpC intergenic region of 97 isoniazid (INH)-resistant and 60 INH-susceptible Mycobacterium tuberculosis isolates obtained in two states in Brazil: São Paulo and Parana. PCR-single-strand conformational polymorphism (PCR-SSCP) was evaluated for screening mutations in regions of prevalence, including codons 315 and 463 of katG, the regulatory region and codons 16 and 94 of inhA, kasA, and the oxyR-ahpC intergenic region. DNA sequencing of PCR amplicons was performed for all isolates with altered PCR-SSCP profiles. Mutations in katG were found in 83 (85.6%) of the 97 INH-resistant isolates, including mutations in codon 315 that occurred in 60 (61.9%) of the INH-resistant isolates and 23 previously unreported katG mutations. Mutations in the inhA promoter region occurred in 25 (25.8%) of the INH-resistant isolates; 6.2% of the isolates had inhA structural gene mutations, and 10.3% had mutations in the oxyR-ahpC intergenic region (one, nucleotide -48, previously unreported). Polymorphisms in the kasA gene occurred in both INH-resistant and INH-susceptible isolates. The most frequent polymorphism encoded a G(269)A substitution. Although KatG(315) substitutions are predominant, novel mutations also appear to be responsible for INH resistance in the two states in Brazil. Since ca. 90.7% of the INH-resistant isolates had mutations identified by SSCP electrophoresis, this method may be a useful genotypic screen for INH resistance. | |
dc.language | eng | |
dc.publisher | Amer Soc Microbiology | |
dc.relation | Antimicrobial Agents and Chemotherapy | |
dc.relation | 4.255 | |
dc.relation | 2,291 | |
dc.rights | Acesso aberto | |
dc.source | Web of Science | |
dc.title | Screening and characterization of mutations in isoniazid-resistant Mycobacterium tuberculosis isolates obtained in Brazil | |
dc.type | Artigo | |